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Field avian metapneumovirus evolution avoiding vaccine induced immunity.

Published on Jan 1, 2010in Vaccine3.269
· DOI :10.1016/j.vaccine.2009.10.149
Elena Catelli13
Estimated H-index: 13
(UNIBO: University of Bologna),
C. Lupini3
Estimated H-index: 3
(UNIBO: University of Bologna)
+ 3 AuthorsClive J. Naylor18
Estimated H-index: 18
(University of Liverpool)
Abstract
Live avian metapneumovirus (AMPV) vaccines have largely brought turkey rhinotracheitis (TRT) under control in Europe but unexplained outbreaks still occur. Italian AMPV longitudinal farm studies showed that subtype B AMPVs were frequently detected in turkeys some considerable period after subtype B vaccination. Sequencing showed these to be unrelated to the previously applied vaccine. Sequencing of the entire genome of a typical later isolate showed numerous SH and G protein gene differences when compared to both a 1987 Italian field isolate and the vaccine in common use. Experimental challenge of vaccinated birds with recent virus showed that protection was inferior to that seen after challenge with the earlier 1987 isolate. Field virus had changed in key antigenic regions allowing replication and leading to disease in well vaccinated birds.
  • References (27)
  • Citations (37)
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References27
Newest
#1Caroline Banet-Noach (TAU: Tel Aviv University)H-Index: 14
#2Lubov SimanovH-Index: 9
Last. Eran Bacharach (TAU: Tel Aviv University)H-Index: 23
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Abstract Avian metapneumovirus (aMPV) is a respiratory pathogen and includes four subtypes, which are differentially distributed worldwide. In Israel, two aMPV subtypes, A and B, are cocirculating in an area with a relatively high density of poultry houses. We performed a longitudinal survey in commercial flocks of turkeys and chickens (six and two flocks, respectively) with differing vaccination programs. This study revealed that all monitored flocks were infected by aMPV during the sampling pe...
16 CitationsSource
#1M. CecchinatoH-Index: 1
#2Elena CatelliH-Index: 13
Last. E. F. KaletaH-Index: 2
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#1Miranda de Graaf (EUR: Erasmus University Rotterdam)H-Index: 25
#2Albert D. M. E. Osterhaus (EUR: Erasmus University Rotterdam)H-Index: 113
Last. Edward C. Holmes (PSU: Pennsylvania State University)H-Index: 113
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Human (HMPV) and avian (AMPV) metapneumoviruses are closely related viruses that cause respiratory tract illnesses in humans and birds, respectively. Although HMPV was first discovered in 2001, retrospective studies have shown that HMPV has been circulating in humans for at least 50 years. AMPV was first isolated in the 1970s, and can be classified into four subgroups, A–D. AMPV subgroup C is more closely related to HMPV than to any other AMPV subgroup, suggesting that HMPV has emerged from AMPV...
69 CitationsSource
#1Roger Ling (Warw.: University of Warwick)H-Index: 9
#2Sabrina Sinkovic (Warw.: University of Warwick)H-Index: 1
Last. Andrew J. Easton (Warw.: University of Warwick)H-Index: 39
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Subgroup A avian metapneumoviruses lacking either the SH or G gene or the M2-2 open reading frame were generated by using a reverse-genetics approach. The growth properties of these viruses were studied in vitro and in vivo in their natural host. Deletion of the SH gene alone resulted in the generation of a syncytial-plaque phenotype and this was reversed by the introduction of the SH gene from a subgroup B, but not a subgroup C, virus. Infected turkeys were assessed for antibody production and ...
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#1Abinash Padhi (TU: University of Tulsa)H-Index: 14
#2Bindhu Verghese (TU: University of Tulsa)H-Index: 8
Abstract Human metapneumovirus (hMPV), a newly discovered virus of the family Paramyxoviridae , has been associated with upper and lower respiratory tract infections in different age groups in many countries. The putative attachment (G) glycoprotein of this virus was previously reported to have shown more extensive nucleotide and deduced amino acid sequence polymorphism than any other genomic regions of this virus, leading to four sub-lineages. Using a maximum likelihood-based codon substitution...
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#1Clive J. Naylor (University of Liverpool)H-Index: 18
#2Roger Ling (Warw.: University of Warwick)H-Index: 9
Last. Andrew J. Easton (Warw.: University of Warwick)H-Index: 39
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A prototype avian metapneumovirus (AMPV) vaccine (P20) was previously shown to give variable outcomes in experimental trials. Following plaque purification, three of 12 viruses obtained from P20 failed to induce protection against virulent challenge, whilst the remainder retained their protective capacity. The genome sequences of two protective viruses were identical to the P20 consensus, whereas two non-protective viruses differed only in the SH gene transcription termination signal. Northern b...
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#1Elena Catelli (UNIBO: University of Bologna)H-Index: 13
#2Cecchinato Mattia (UNIBO: University of Bologna)H-Index: 6
Last. Clive J. Naylor (University of Liverpool)H-Index: 18
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A live A type avian metapneumovirus (AMPV) vaccine which had been shown to be highly protective and short lived in experimental conditions was found to persist for longer periods in the field and to be associated with disease. Previously other factors such as possible secondary pathogens and management considerations had made it impossible to conclude whether the observed disease was a result of an increase in the vaccine virulence. In this study, an AMPV was isolated from poults on a farm which...
60 CitationsSource
#2Lubov SimanovH-Index: 9
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Although the disease associated with the avian metapneumovirus (aMPV) has been described in Israel for more than 20 years and one Israeli isolate was characterized as subtype A, this is the first study investigating the strains circulating in Israel (period 2002 to 2004). Viral RNA was detected by reverse transcription-polymerase chain reaction in 44% of the examined flocks, and one-half of the virus isolation attempts were successful. Characterization showed that 34 non-vaccinated turkey flocks...
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#1Clive J. Naylor (University of Liverpool)H-Index: 18
#2P. Brown (University of Liverpool)H-Index: 4
Last. Andrew J. Easton (Warw.: University of Warwick)H-Index: 39
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Avian pneumovirus (APV) is a member of the genus Metapneumovirus of the subfamily Pneumovirinae. This study describes the development of a reverse-genetics system for APV. A minigenome system was used to optimize the expression of the nucleoprotein, phosphoprotein, M2 and large polymerase proteins when transfected into Vero cells under the control of the bacteriophage T7 promoter. Subsequently, cDNA was transcribed from the virion RNA to make a full-length antigenome, which was also cloned under...
51 CitationsSource
Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99·7% nt and 99·0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70·0–80·5% and 77·6–97·2%, respectively, with the L gene sharing 76·1% nt and 85·3% aa identity with that of a...
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