Chromatography

Gas chromatography–mass spectrometry

Squalene

Biochemistry

Phytochemical

Alternative medicine

Organic chemistry

Triterpene

Gas chromatography

Medicine

Mass spectrometry

Chemistry

Polygonum

Ethanol

Traditional medicine

Pathology

Pharmacognosy Research

Pharmacognosy Research,2012,4,1,11-14.DOI:10.4103/0974-8490.91028Published:december,2011Type:Original ArticleAuthors:Bagavathi Perumal Ezhilan, and Ramasamy Neelamegam Author(s) affiliations:Bagavathi Perumal Ezhilan, Ramasamy Neelamegam Department of Botany and Research Centre, S.T. Hindu College, Nagercoil-629 002, Kanyakumari (Dist.), Tamil Nadu, India. Abstract:Background: The presence of diverse secondary metabolites has been reported from species of the genus Polygonum. However, there has been not much information available on phytochemical components and biological activity in the whole plant ethanol extract of Polygonum chinense L. Objective: This study was designed to determine the phytocomponents in the whole plant ethanol extract of P. chinense. Materials and Methods: GC-MS analysis of the whole plant ethanol extract of P. chinense was performed using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i auto-sampler and a gas chromatograph interfaced to a mass spectrometer (GC-MS). Results: This investigation was carried out to determine the possible chemical components from P. chinense by GC-MS. This analysis revealed that the ethanol extract of P. chinense (whole plant) contained mainly a triterpene compound-squalene (47.01%), and a plasticizer compound-1,2-benzenedicarboxylic acid, mono[2-ethylhexyl]ester (40.30%). All identified compounds were, generally, reported as having antimicrobial activity. In addition, the squalene compound also having anti-cancer, anti-oxidant, anti-tumor, chemo-preventive, pesticidal and sun-screen properties, while the plasticizer compound -1,2-benzenedicarboxylic acid, mono[2-ethylhexyl] ester reported to have anti-oxidant and anti-inflammatory properties. No activity was reported in the alcoholic compound-4-hexene-1-ol, 5-methyl-2-(1-methylethanyl)-acetate-(R)-. Conclusions: From the results, it is evident that P. chinense contains various bioactive compounds and is recommended as a plant of phytopharmaceutical importance. Keywords:GC-MS Analysis, Polygonum chinense, Rhytocomponents, Whole plant ethanol extractView:PDF (1.31 MB)

GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L.