The intracellular oxidation of 2′,7′-dichlorofluorescin in murine T lymphocytes
Abstract
Intracellular reactive oxygen species (ROS) production by activated murine T lymphocytes was investigated by analyzing intracellular dichlorofluorescin (DCFH2) oxidation in lymph node cells (LNC). An increase in DCFH2 oxidation in LNC induced by phorbol myristate acetate (PMA) was detected by flow cytometry. It was confirmed that this increase was present in Thy1+ LNC. We examined the contribution to intracellular DCFH2 oxidation of ROS released...
Paper Details
Title
The intracellular oxidation of 2′,7′-dichlorofluorescin in murine T lymphocytes
Published Date
Jan 1, 2001
Volume
30
Issue
1
Pages
82 - 88
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