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Structure of yeast phenylalanine tRNA at 3 A resolution.

Published on Aug 1, 1974in Nature43.07
· DOI :10.1038/250546a0
Jon D. Robertus43
Estimated H-index: 43
,
Jane E. Ladner18
Estimated H-index: 18
(Medical Research Council)
+ 4 AuthorsA. Klug1
Estimated H-index: 1
(Medical Research Council)
Abstract
The structure of a tRNA has been determined by isomorphous replacement. Some of the interactions which maintain the tertiary structure are of a novel type. Our model differs significantly from one which has recently been proposed.
  • References (23)
  • Citations (772)
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Abstract The bases of yeast tRNAPhe which react with carbodiimide and methoxyamine have been determined and this information has been combined with chemical modification studies of other workers to produce a composite picture of base accessibility in this tRNA. The results are compared with the three-dimensional structure which we have recently determined. The bases which react chemically lie in exposed positions in the three-dimensional model and those which do not are either in the double heli...
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#1F. L. Suddath (MIT: Massachusetts Institute of Technology)H-Index: 12
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At 3A resolution, the electron density map of crystalline tRNA shows the polynucleotide chain as an alternating series of ribose and phosphate peaks. Bases are seen, especially in the double helical stem regions. A complete three-dimensional model of the L-shaped molecule has been built.
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#1Michael Levitt (Weizmann Institute of Science)H-Index: 99
Abstract A search of the X-ray intensities of the P 2 1 crystal form of yeast transfer RNA Phe has revealed the orientation of the double-helical segments in the crystal. Because of the ambiguity imposed by the crystal symmetry on choosing the helices belonging to the same molecule, and because of the difficulty of determining lengths and positions of helices, a unique model cannot be deduced, but only a small number of types are possible. Among the possibilities are a “boot”-shaped molecule, wh...
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Systematic crystallization studies on yeast transfer RNAphe have yielded crystals large enough for X-ray crystallography. The crystal form is monoclinic; P21 a = 56.0 A, b = 33.4 A, c = 63.0 A and β = 90.4 °. The X-ray diffraction patterns extend to beyond 3 A spacings.
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Three isomorphous heavy-atom derivatives have been obtained of orthorhombic crystals of phenylalanine transfer RNA from yeast. These derivatives contain osmium, samarium, and platinum. The positions of the heavy atoms have been determined; these have been used to calculate a three-dimensional electron-density map of transfer RNA at a resolution of 5.5 A. The map shows a high contrast between the molecular boundaries and the solvent areas, so that most of the external shape of the molecule can be...
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