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Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection

Published on Jun 1, 1997in Journal of Chromatography B: Biomedical Sciences and Applications
· DOI :10.1016/S0378-4347(97)00033-9
Chung-Shi Yang13
Estimated H-index: 13
(PC: Providence College),
Pi-Ju Tsai11
Estimated H-index: 11
+ 3 AuthorsJon-Son Kuo26
Estimated H-index: 26
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Abstract
Abstract A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion and high-performance liquid chromatography. The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively). Lipid peroxidation in the extracellular space was evaluated as the concentration of malondialdehyde, a lipid peroxidation end product which reacts with TBA to form a fluorescent conjugate. Significantly increased production of malondialdehyde following hydrogen peroxide perfusion (0.03%, 0.3% at a flow-rate of 1 μl/min) was observed in the brain cortex of anaesthetized rats.
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  • Citations (12)
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References31
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#1A.H. Waterfall (University of Nottingham)H-Index: 3
#2G. Singh (University of Nottingham)H-Index: 2
Last.Charles A. Marsden (University of Nottingham)H-Index: 60
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#1Ewa Sewerynek (University of Texas Health Science Center at San Antonio)H-Index: 22
#2Burkhard Poeggeler (University of Texas Health Science Center at San Antonio)H-Index: 43
Last.Russel J. Reiter (University of Texas Health Science Center at San Antonio)H-Index: 143
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#1Domenico Alvaro (Sapienza University of Rome)H-Index: 53
#2Alessandro Gigliozzi (Sapienza University of Rome)H-Index: 12
Last.L. Capocaccia (Sapienza University of Rome)H-Index: 36
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#1Roberto Vagnozzi (University of Rome Tor Vergata)H-Index: 25
#2Giuseppe Lazzarino (University of Rome Tor Vergata)H-Index: 38
Last.Bruno Giardina (Sapienza University of Rome)H-Index: 46
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Cited By12
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#1Ying Wang (Fudan University)H-Index: 1
#2Carole Calas-Blanchard (University of Perpignan)H-Index: 12
Last.Jean-Louis Marty (University of Perpignan)H-Index: 27
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