Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection

Published on Jun 1, 1997in Journal of Chromatography B: Biomedical Sciences and Applications
· DOI :10.1016/S0378-4347(97)00033-9
Chung-Shi Yang13
Estimated H-index: 13
(PC: Providence College),
Pi-Ju Tsai11
Estimated H-index: 11
+ 3 AuthorsJon-Son Kuo26
Estimated H-index: 26
Abstract A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion and high-performance liquid chromatography. The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively). Lipid peroxidation in the extracellular space was evaluated as the concentration of malondialdehyde, a lipid peroxidation end product which reacts with TBA to form a fluorescent conjugate. Significantly increased production of malondialdehyde following hydrogen peroxide perfusion (0.03%, 0.3% at a flow-rate of 1 μl/min) was observed in the brain cortex of anaesthetized rats.
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