Controlled Cell Adhesion Using a Biocompatible Anchor for Membrane-Conjugated Bovine Serum Albumin/Bovine Serum Albumin Mixed Layer

We report here a method for controlling cell adhesion, allowing simple yet accurate cell detachment from the substrate, which is required for the establishment of new cytometry-based cell processing and analyzing methods. A biocompatible anchor for membrane (BAM) was conjugated with bovine serum albumin (BSA) to produce a cell-anchoring agent (BAM–BSA). By coating polystyrene substrates with a mixture of BAM–BSA and BSA, controlled suppression...