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The science of macromolecular crystallization

Published on Aug 1, 1995in Structure4.576
· DOI :10.1016/S0969-2126(01)00211-8
Alexander McPherson51
Estimated H-index: 51
(UCR: University of California, Riverside),
A Malkin18
Estimated H-index: 18
(UCR: University of California, Riverside),
Yurii G. Kuznetsov4
Estimated H-index: 4
(UCR: University of California, Riverside)
Abstract
Thanks to Bob Cudney, Hampton Research, Laguna Hills, CA, USA for kindly providing Figure 11Figure 11. The research described here was supported by grants and contracts from the National Aeronautics and Space Administration.
  • References (85)
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The evolution of surface morphology and step dynamics during growth of rhombohedral crystals of the protein canavalin and crystals of the cubic satellite tobacco mosaic virus (STMV) have been investigated for the first time by in situ atomic force microscopy. These two crystals were observed to grow by very different mechanisms. Growth of canavalin occurs on complex vicinal hillocks formed by multiple, independently acting screw dislocations. Small clusters were observed on the terraces. STMV on...
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Abstract In situ laser Michelson interferometry was utilized to investigate the growth kinetics and surface morphology in canavalin, thaumatin, and turnip yellow mosaic virus (TYMV) crystallization. Interferometric patterns and kinetic measurements from growing macromolecular crystals as small as 20 μm were obtained. This study shows that for the crystallization of canavalin, dislocations are the sources of growth steps on the surfaces of growing crystals. Supersaturation dependencies of the nor...
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Attempts were made to crystallize four monoclonal antibodies, one IgG2ak and three IgG1k. Using a PEG 3350 screen combined with detergents, and developed from our experiments with an IgG2ak antibody specific for canine lymphoma cells,1,2 crystals have now been obtained of two of these four immunoglobulins, an antiphenytoin and an antiphenobarbital antibody. A complex between the antiphenobarbital antibody and its drug antigen crystallized as well. The antibody for phenytoin has, to this point, p...
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Abstract In situ laser Michelson interferometry was utilized to investigate mechanisms of growth and surface morphology in protein and virus crystallization, These included plant proteins canavalin and thaumatin and turnip yellow mosaic virus. The experimental apparatus allowed us to obtain interferometric patterns and investigate growth kinetics from growing macromolecular crystals as small as 20 μm. For the crystallization of canavalin, dislocations are the sources of growth steps on the surfa...
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