A novel real‐time CTL assay to measure designer T‐cell function against HIV Env⁺ cells

Background To increase the immunosurveillance in HIV infection, we used retroviral vectors expressing CD 4‐chimeric antigen receptors ( CAR s) to genetically modify autologous T cells and redirect CTL toward HIV . The CD 4 extracellular domain targets envelope and the intracellular signaling domains activate T cells. The maC46 fusion inhibitor binds HIV and blocks viral replication. Methods We stimulated rhesus PBMC s with antibodies to CD3/CD28...