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Protocatechuic acid inhibits apoptosis by mitochondrial dysfunction in rotenone-induced PC12 cells.

Published on Mar 1, 2008in Toxicology in Vitro 3.07
· DOI :10.1016/j.tiv.2007.10.012
Ye-Ming Liu1
Estimated H-index: 1
(DUT: Dalian University of Technology),
Bo Jiang19
Estimated H-index: 19
(DUT: Dalian University of Technology)
+ 1 AuthorsLijia An16
Estimated H-index: 16
(DUT: Dalian University of Technology)
Cite
Abstract
Protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia (A.) oxyphylla, showed the significant neuroprotective effects on hydrogen peroxide (H2O2) or MPP+-induced apoptosis in cultured PC12 cells. However, the mechanism how PCA suppresses rotenone-induced neurotoxicity in cultured PC12 cells remains unclear. In this study, we investigated the protective effects of PCA in PC12 cells exposed to rotenone as an in vitro model of mitochondrial dysfunction and apoptotic cell death. The apoptosis in rotenone-induced PC12 cells was accompanied by the loss of mitochondrial membrane potential, the formation of reactive oxygen species (ROS), the total glutathione depletion, activation of caspase-3 and down-regulation of Bcl-2. In contrast, PCA markedly attenuated the above-mentioned mitochondrial dysfunction in a dose-dependent manner. Taken together, these results suggest that treatment of PC12 cells with PCA can block rotenone-induced apoptosis via ameliorating the mitochondrial dysfunction.
  • References (37)
  • Citations (43)
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References37
Newest
Published on May 1, 2007in Environmental Toxicology and Pharmacology 3.06
Yu-Rong Mao1
Estimated H-index: 1
(DUT: Dalian University of Technology),
Lan Jiang2
Estimated H-index: 2
(NU: Northeastern University)
+ 2 AuthorsBo Jiang2
Estimated H-index: 2
(DUT: Dalian University of Technology)
Abstract Rotenone, a specific inhibitor of mitochondrial complex I, reproduces many features of Parkinson's disease. The aim of the study was carried out to investigate how rotenone affected the mitochondrial function and antioxidant/oxidant parameters of mouse striatum, and secondly, to evaluate the ameliorating effects of catalpol against rotenone-induced damage. Our results showed that rotenone induced significant changes in mitochondrial function such as complex I activity and mitochondrial ...
Published on Jan 1, 2007in Methods in Cell Biology 1.70
Naoufal Zamzami21
Estimated H-index: 21
(Institut Gustave Roussy),
Carine Maisse6
Estimated H-index: 6
(Institut Gustave Roussy)
+ 1 AuthorsGuido Kroemer191
Estimated H-index: 191
(Institut Gustave Roussy)
Publisher Summary This chapter focuses on the experimental procedures designed to measure apoptosis-associated mitochondrial membrane permeabilization, either in intact cells or in isolated mitochondria. Following a variety of death signals, mitochondria show early alterations in their function that at least in some instances may be explained by the opening of the permeability transition (PT) pore. The PT pore—also called mitochondrial megachannel or multiple conductance channels—is a dynamic mu...
Published on Oct 1, 2006in Food and Chemical Toxicology 3.77
Shui Guan10
Estimated H-index: 10
(DUT: Dalian University of Technology),
Bo Jiang19
Estimated H-index: 19
(DUT: Dalian University of Technology)
+ 1 AuthorsLijia An16
Estimated H-index: 16
(DUT: Dalian University of Technology)
Protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia (A.) oxyphylla, showed antioxidant neuroprotective effect in our previous study. Here, we investigated the effect of PCA on the MPP+-induced mitochondrial dysfunction and apoptotic cell death in PC12 cells. The apoptosis in MPP+-induced PC12 cells was associated with loss of mitochondrial membrane potential, the formation of reactive oxygen species (ROS), GSH depletion, activation of caspase-3 and down-regulation...
Published on Aug 1, 2006in Neuroscience Letters 2.17
Gui-Fang Shi3
Estimated H-index: 3
(DUT: Dalian University of Technology),
Lijia An5
Estimated H-index: 5
(DUT: Dalian University of Technology)
+ 2 AuthorsYongming Bao17
Estimated H-index: 17
(DUT: Dalian University of Technology)
Abstract In this study, the neuroprotective effects of Alpinia protocatechuic acid (PCA), a phenolic compound isolated from the dried fruits of Alpinia Oxyphylla Miq. was found. The protective effect of Alpinia PCA against H 2 O 2 -induced oxidative damage on PC12 cells was investigated by measuring cell viability via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Rats were injected intraperitoneally with Alpinia PCA at a dose of 5 mg/k...
Published on May 1, 2006in European Journal of Pharmacology 3.17
Shui Guan10
Estimated H-index: 10
(DUT: Dalian University of Technology),
Yongming Bao17
Estimated H-index: 17
(DUT: Dalian University of Technology)
+ 1 AuthorsLijia An5
Estimated H-index: 5
(DUT: Dalian University of Technology)
Abstract The neuroprotective effects of protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia oxyphylla , on hydrogen peroxide (H 2 O 2 )-induced apoptosis and oxidative stress in cultured PC12 cells were investigated. Exposure of PC12 cells to 0.4 mM H 2 O 2 induced a leakage of lactate dehydrogenase (LDH) and decreased cell viability denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. PCA increased PC12 cellular viability and marke...
Published on Mar 1, 2006in Food and Chemical Toxicology 3.77
Lijia An16
Estimated H-index: 16
(DUT: Dalian University of Technology),
Shui Guan10
Estimated H-index: 10
(DUT: Dalian University of Technology)
+ 3 AuthorsBo Jiang19
Estimated H-index: 19
(DUT: Dalian University of Technology)
Abstract An ethyl acetate extract of Alpinia oxyphylla was found to possess neuroprotective activity against 1-methyl-4-phenylpyridinium ion (MPP + ) induced apotosis and oxidative stress in cultured PC12 cells. From the extract, a phenolic compound was isolated through bioassay-guided fractionation and identified as protocatechuic acid (PCA) by IR, MS, and 1 H and 13 C NMR spectroscopy. It was the first time which was isolated from the kernels of A. oxyphylla . Exposure of PC12 cells to 1 mM MP...
Published on Jul 18, 2005in FEBS Letters 2.67
Gang Wang17
Estimated H-index: 17
(Ruijin Hospital),
Chen Qi3
Estimated H-index: 3
(Ruijin Hospital)
+ 2 AuthorsSheng-Di Chen36
Estimated H-index: 36
(Ruijin Hospital)
In vivo and in vitro studies have suggested a neuroprotective role for Pituitary adenylate cyclase activating polypeptide (PACAP) against neuronal insults. Here, we showed that PACAP27 protects against neurotoxicity induced by rotenone, a mitochondrial complex I inhibitor that has been implicated in the pathogenesis of Parkinson's disease (PD). The neuroprotective effect of PACAP27 was dose-dependent and blocked by its specific receptor antagonist, PACAP6-27. The effects of PACAP27 on rotenone-i...
Published on Jul 1, 2005in Brain Research 2.93
Karuppagounder S. Saravanan11
Estimated H-index: 11
(IICB: Indian Institute of Chemical Biology),
Kizhakke M. Sindhu8
Estimated H-index: 8
(IICB: Indian Institute of Chemical Biology),
Kochupurackal P. Mohanakumar30
Estimated H-index: 30
(IICB: Indian Institute of Chemical Biology)
Abstract We examined in Sprague–Dawley rats whether intranigral administration of complex-I inhibitor, rotenone, produces biochemical lesions in the striatum similar to those observed in Parkinson's disease (PD). Unilateral stereotaxic infusion of rotenone (2–12 μg in 1 μl) into substantia nigra (SN) pars compacta caused significant inhibition of complex-I activity and increased production of hydroxyl radicals in vivo as measured employing spectrophotometric and HPLC-electrochemical procedures, ...
Published on Sep 1, 2004in Journal of Neurochemistry 4.87
Brian M. Polster8
Estimated H-index: 8
(Johns Hopkins University),
Gary Fiskum63
Estimated H-index: 63
(UMB: University of Maryland, Baltimore)
The importance of calcium overload, mitochondrial dysfunction, and free radical generation to neuropathological processes has been recognized for many years. Only more recently has evidence accumulated that the programmed cell death process of apoptosis plays an integral role not only in the development of the nervous system, but in the loss of cells following acute neurological insults and chronic disease. In 1996 came the landmark discovery that cytochrome c, an evolutionary old and essential ...
Published on Nov 26, 2003in The Journal of Neuroscience 6.07
Todd B. Sherer21
Estimated H-index: 21
(Emory University),
Ranjita Betarbet24
Estimated H-index: 24
(Emory University)
+ 7 AuthorsJ. Timothy Greenamyre52
Estimated H-index: 52
(Emory University)
Exposure of rats to the pesticide and complex I inhibitor rotenone reproduces features of Parkinson9s disease, including selective nigrostriatal dopaminergic degeneration and α-synuclein-positive cytoplasmic inclusions (Betarbet et al., 2000; Sherer et al., 2003). Here, we examined mechanisms of rotenone toxicity using three model systems. In SK-N-MC human neuroblastoma cells, rotenone (10 nm to 1 μm) caused dose-dependent ATP depletion, oxidative damage, and death. To determine the molecular si...
Cited By43
Newest
Published in Antioxidants
Aimee N. Winter7
Estimated H-index: 7
,
Paula C. Bickford
Neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS), are characterized by the death of neurons within specific regions of the brain or spinal cord. While the etiology of many neurodegenerative diseases remains elusive, several factors are thought to contribute to the neurodegenerative process, such as oxidative and nitrosative stress, excitotoxicity, endoplasmic reticulum stress, protein aggregation, and neuroinflammation. These...
Published in Nutrients 4.17
Benjamin Dilberger1
Estimated H-index: 1
,
Maike Passon + -3 AuthorsGunter P. Eckert41
Estimated H-index: 41
(1) Background: Polyphenols (PP) play an important role in the prevention of non-communicable diseases and may contribute to healthy aging. To investigate the molecular and cellular aspects of PP metabolites on longevity with a focus on mitochondrial function, we applied a pre-fermented mixture of polyphenols (Rechtsregulat®, RR) to rodents and nematodes. (2) Methods: The lifespans of Navar Medical Research Institute (NMRI) mice and C. elegans were recorded. The heat-stress resistance (37 °C) of...
Published on May 1, 2019in Plant Cell Tissue and Organ Culture 2.20
Claude Y. Hamany Djande (UJ: University of Johannesburg), Paul A. Steenkamp15
Estimated H-index: 15
(UJ: University of Johannesburg)
+ 2 AuthorsIan A. Dubery28
Estimated H-index: 28
(UJ: University of Johannesburg)
Undifferentiated plant cells in culture represent a renewable system conducive to understanding biological processes and a valuable alternative for secondary metabolite production. Additionally, manipulation of these systems by plant growth regulators (PGRs) may result in redifferentiation/organogenesis and hence changes in metabolic profiles. The aim of the study was to investigate the effects of combining auxin (2,4-dichlorophenoxyacetic acid) and cytokinin (kinetin) at concentrations of 2, 4,...
Published on Jan 23, 2019in Nutrients 4.17
Hung-Chi Chang6
Estimated H-index: 6
,
Keng-Fan Liu + 4 AuthorsChi-Rei Wu23
Estimated H-index: 23
Sophora species are used as dietary medicines in aging-associated symptoms. Sophora tomentosa L. (ST) is a native medicinal plant in Southeast Asia; however, there is no pharmacological literature about ST extract. The present study evaluates the antioxidant phytoconstituent contents and radical scavenging capacities of ST extract. The further investigation was to clarify the neuroprotective mechanism of ST extract against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism ...
Published on Nov 1, 2018in Microbial Pathogenesis 2.58
Changbo Ou3
Estimated H-index: 3
,
Qiuxia Wang2
Estimated H-index: 2
+ 3 AuthorsCheng He5
Estimated H-index: 5
(CAU: China Agricultural University)
Abstract Infectious bursal disease virus (IBDV) is a very important small RNA virus in the family of Birnaviridae, which can cause severe immunosuppressive effects and pathological damages in young chickens. It can replicate in bursal lymphocytes and impede the growth and development of B cells, finally causing bursal lymphocytes apoptosis. Previous results have shown that protocatechuic acid (PCA) as an important phenolic compound could effectively improve the survival rate of chickens infected...
Published on Oct 1, 2018in Journal of Ethnopharmacology 3.41
Ibrahim Babangida Abubakar5
Estimated H-index: 5
,
Ibrahim Malami3
Estimated H-index: 3
(Uthman dan Fodiyo University)
+ 1 AuthorsSahabi Manga Sule1
Estimated H-index: 1
Abstract Ethnopharmacological relevance Alpinia officinarum Hance is a perennial plant that has been traditionally used for many decades to treat several ailments including inflammation, pain, stomach-ache, cold, amongst others. Pharmacological studies over the years have demonstrated remarkable bioactivities that could be further explored for development of new therapeutic agents against various ailments. Aim of the study The paper critically reviewed the ethno-medicinal uses, pharmacology, and...
Published on Sep 1, 2018in Fitoterapia 2.43
Ya-Qian Ma3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences),
Menghan Liu2
Estimated H-index: 2
(NCU: Nanchang University)
+ 4 AuthorsChang-Heng Tan12
Estimated H-index: 12
(CAS: Chinese Academy of Sciences)
Abstract The fruits of Swietenia macrophylla (skyfruits) are commercially used as healthcare products to improve blood circulation. An investigation of active ingredients of skyfruits led to the isolation of four new limonoids, swietemacrolides A−D ( 1 – 4 ), together with ten known limonoids ( 5 – 14 ) and one proto-limonoid ( 15 ). Their structures were elucidated on the basis of MS and NMR data analysis. Swietemacrolide C ( 3 ) at the concentration of 10 μM showed significant protective effec...
Published on Apr 1, 2018in International Journal of Biological Macromolecules 4.78
Chao Xu2
Estimated H-index: 2
(DUT: Dalian University of Technology),
Shui Guan10
Estimated H-index: 10
(DUT: Dalian University of Technology)
+ 5 AuthorsTianqing Liu20
Estimated H-index: 20
(DUT: Dalian University of Technology)
Abstract Excessive free radicals can cause oxidative damage to human tissues, which results in a variety of diseases. Therefore, the development of antioxidant materials is one of the great projects in biomedical field. In this work, antioxidant protocatechuic acid (PCA) monomers were grafted onto chitosan (CS) backbones to develop a PCA grafted chitosan (PCA-g-CS) antioxidant copolymer via the method of free radical-induced grafting reaction. The formation of covalent bonds between PCA and CS w...
Published on Dec 11, 2017in Antioxidants & Redox Signaling 5.83
F StevensJan1
Estimated H-index: 1
,
S RevelJohana1
Estimated H-index: 1
,
S MaierClaudia1
Estimated H-index: 1
Abstract Significance: Humans are exposed daily to polyphenols in milligram-to-gram amounts through dietary consumption of fruits and vegetables. Polyphenols are also available as components of dietary supplements for improving general health. Although polyphenols are often advertised as antioxidants to explain health benefits, experimental evidence shows that their beneficial cancer preventing and controlling properties are more likely due to stimulation of pro-oxidant and proapoptotic pathways...
Published on Dec 1, 2017in Scientific Reports 4.01
José Teixeira8
Estimated H-index: 8
(UC: University of Coimbra),
Catarina Oliveira4
Estimated H-index: 4
(University of Porto)
+ 8 AuthorsPaulo J. Oliveira50
Estimated H-index: 50
(UC: University of Coimbra)
Oxidative stress and mitochondrial dysfunction have been associated with metabolic and age-related diseases. Thus, the prevention of mitochondrial oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between the consumption of polyphenol-rich diet and the prevention of different pathologies, including diseases with a mitochondrial etiology. The development of mitochondrial-targeted antioxidants based on ...