TALENs: a widely applicable technology for targeted genome editing

Transcription activator-like effector nucleases (TALENs) comprise a nonspecific nuclease fused to a sequence-specific DNA-binding domain. This domain can be engineered so that TALENs can target virtually any sequence. TALENs are an efficient tool to modify genes in a wide range of cell types and organisms. Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed...