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Sparse matrix sampling: a screening method for crystallization of proteins

Published on Aug 1, 1991in Journal of Applied Crystallography
· DOI :10.1107/S0021889891004430
Jaru Jancarik18
Estimated H-index: 18
,
Sung-Hou Kim83
Estimated H-index: 83
Abstract
A set of screening conditions for initial experiments in protein crystallization has been developed, tested, and is herein presented. These solution and precipitant conditions are empirically derived based on known or published crystallization conditions of various proteins in the past, so as to sample as large a range of buffer, pH, additive and precipitant variables as possible, using small amounts of proteins. The 50 crystallization conditions have been tested on 15 previously crystallized proteins, all of which were also crystallized in at least one form by this screen. This method is also shown to be highly successful in the crystallization of proteins which had not previously been crystallized.
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Cited By1890
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#1Valentina Corvaglia (LMU: Ludwig Maximilian University of Munich)
#2Daniel Carbajo (University of Bordeaux)
Last.Ivan Huc (LMU: Ludwig Maximilian University of Munich)H-Index: 47
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#1Vera V. Nazarenko (MIPT: Moscow Institute of Physics and Technology)
#2V.V. Nazarenko (MIPT: Moscow Institute of Physics and Technology)
Last.Ivan Yu. Gushchin (MIPT: Moscow Institute of Physics and Technology)H-Index: 10
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#1Kengo Kitadokoro (Kyoto Institute of Technology)H-Index: 7
#2Mizuki Kakara (Kyoto Institute of Technology)H-Index: 1
Last.Shigeki Kamitani (OPU: Osaka Prefecture University)H-Index: 2
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