Purification and Properties of Phosphoenolpyruvate and Ribulose Diphosphate Carboxylases from C4 and C3 Plants

Ribulose diphosphate (RuDP) and phosphoenolpyruvate (PEP) carboxylases were partially purified from leaves of three C4 plants: Setaria, Pennisetum and Amaranthus and two C3 plants: Oryza and Rumex. RuDP carboxylase from C4 plants had a lower Km for RuDP and HCO3− than that of the enzyme from C3 type. Their reaction velocities were similar in all cases, reflecting the potential of RuDP carboxylase in C4 plants as well as in C3 plants. PEP...