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Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium

Published on Jan 17, 2006in Proceedings of the National Academy of Sciences of the United States of America9.58
· DOI :10.1073/pnas.0504860102
Naomi E. Chayen33
Estimated H-index: 33
,
Emmanuel N. Saridakis52
Estimated H-index: 52
,
Richard P. Sear29
Estimated H-index: 29
Abstract
The determination of high-resolution structures of proteins requires crystals of suitable quality. Because of the new impetus given to structural biology by structural genomics/proteomics, the problem of crystallizing proteins is becoming increasingly acute. There is therefore an urgent requirement for the development of new efficient methods to aid crystal growth. Nucleation is the crucial step that determines the entire crystallization process. Hence, the holy grail is to design a “universal nucleant,” a substrate that induces the nucleation of crystals of any protein. We report a theory for nucleation on disordered porous media and its experimental testing and validation using a mesoporous bioactive gel-glass. This material induced the crystallization of the largest number of proteins ever crystallized using a single nucleant. The combination of the model and the experimental results opens up the scope for the rational design of nucleants, leading to alternative means of controlling crystallization.
  • References (16)
  • Citations (156)
References16
Newest
#1Giuseppe Falini (UNIBO: University of Bologna)H-Index: 36
#2Simona Fermani (UNIBO: University of Bologna)H-Index: 16
Last.Alberto Ripamonti (UNIBO: University of Bologna)H-Index: 27
view all 4 authors...
#1C. Kinowski (CNRS: Centre national de la recherche scientifique)H-Index: 5
#2M. BouazaouiH-Index: 15
Last.Sylvia Turrell (CNRS: Centre national de la recherche scientifique)H-Index: 18
view all 6 authors...
#1Naomi E. Chayen (Imperial College London)H-Index: 33
#2Emmanuel N. Saridakis (Imperial College London)H-Index: 52
Last.Yael Nemirovsky (Technion – Israel Institute of Technology)H-Index: 34
view all 4 authors...
Cited By156
Newest
#1S. Engilberge (CNRS: Centre national de la recherche scientifique)H-Index: 6
#2Tristan Wagner (MPG: Max Planck Society)H-Index: 7
Last.Eric Girard (CNRS: Centre national de la recherche scientifique)H-Index: 20
view all 9 authors...
#1Wester de Poel (Radboud University Nijmegen)H-Index: 3
#2Johannes A. A. W. Elemans (Radboud University Nijmegen)H-Index: 33
Last.Elias Vlieg (Radboud University Nijmegen)H-Index: 19
view all 5 authors...
#1Mark A. Holden (University of Leeds)H-Index: 10
#2Thomas F. Whale (University of Leeds)H-Index: 14
Last.Hugo K. Christenson (University of Leeds)H-Index: 41
view all 8 authors...
#1Sherif Ashraf Fahmy (The: American University in Cairo)H-Index: 1
#2Mohamad Alawak (University of Marburg)
Last.Mayyada M. H. El Sayed (The: American University in Cairo)H-Index: 1
view all 5 authors...
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