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Birth of healthy offspring following ICSI in in vitro-matured common marmoset (Callithrix jacchus) oocytes.

Published on Apr 21, 2014in PLOS ONE2.78
· DOI :10.1371/journal.pone.0095560
Tsukasa Takahashi1
Estimated H-index: 1
(Central Institute for Experimental Animals),
Kisaburo Hanazawa10
Estimated H-index: 10
(Juntendo University)
+ 11 AuthorsErika Sasaki20
Estimated H-index: 20
(Central Institute for Experimental Animals)
Abstract
Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ICSI. To investigate the optimal timing of performing ICSI, corrected immature oocytes were matured in vitro and ICSI was performed at various time points (1–2 h, 2–4 h, 4–6 h, 6–8 h, and 8–10 h after extrusion of the first polar body (PB)). Matured oocytes were then divided randomly into two groups: one was used for in vitro fertilization (IVF) and the other for ICSI. To investigate in vivo development of embryos followed by ICSI, 6-cell- to 8-cell-stage embryos and blastocysts were nonsurgically transferred into recipient marmosets. Although no significant differences were observed in the fertilization rate of blastocysts among ICSI timing after the first PB extrusion, the blastocyst rate at 1–2 h was lowest among groups at 2–4 h, 4–6 h, 6–8 h, and 8–10 h. Comparing ICSI to IVF, the fertilization rates obtained in ICSI were higher than in IVF (p>0.05). No significant difference was noted in the cleaved blastocyst rate between ICSI and IVF. Following the transfer of 37 ICSI blastocysts, 4 of 20 recipients became pregnant, while with the transfer of 21 6-cell- to 8-cell-stage ICSI embryos, 3 of 8 recipients became pregnant. Four healthy offspring were produced and grew normally. These are the first marmoset offspring produced by ICSI, making it an effective fertilization method for marmosets.
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References38
Newest
#1Hideyuki Okano (Keio: Keio University)H-Index: 110
#2Keigo Hikishima (Central Institute for Experimental Animals)H-Index: 14
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 20
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#1Melanie Walls (UWA: University of Western Australia)H-Index: 7
#2Stephen JunkH-Index: 1
Last.Roger Hart (UWA: University of Western Australia)H-Index: 34
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#1Ikuo Tomioka (Central Institute for Experimental Animals)H-Index: 11
#2Tsukasa Takahashi (Central Institute for Experimental Animals)H-Index: 1
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 20
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#1Erika Sasaki (Central Institute for Experimental Animals)H-Index: 20
#2Hiroshi Suemizu (Central Institute for Experimental Animals)H-Index: 21
Last.Tatsuji Nomura (Central Institute for Experimental Animals)H-Index: 34
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#1Eric T. Shinohara (UPenn: University of Pennsylvania)H-Index: 18
#2Joseph M. KaminskiH-Index: 19
Last.Ryuzo Yanagimachi (U.H.: University of Hawaii at Manoa)H-Index: 85
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#1Jianhui Tian (CAU: China Agricultural University)H-Index: 14
#2Zhonghong Wu (CAU: China Agricultural University)H-Index: 12
Last.Chang-Xin Wu (CAU: China Agricultural University)H-Index: 1
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#1Ryota Suganuma (UH: University of Hawaii)H-Index: 6
#2Pawel PelczarH-Index: 19
Last.Stefan Moisyadi (UH: University of Hawaii)H-Index: 18
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Cited By13
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#1Jenna Kropp Schmidt (UW: University of Wisconsin-Madison)H-Index: 1
#2Thaddeus G. Golos (UW: University of Wisconsin-Madison)H-Index: 31
#1Fumiko Seki (Central Institute for Experimental Animals)H-Index: 4
#2Keigo Hikishima (Central Institute for Experimental Animals)H-Index: 14
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
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#1Yoko Kurotaki (Central Institute for Experimental Animals)H-Index: 2
#2Erika Sasaki (Central Institute for Experimental Animals)H-Index: 20
#1Jenna Kropp (UW: University of Wisconsin-Madison)H-Index: 11
#2Andrea Di Marzo (UW: University of Wisconsin-Madison)H-Index: 1
Last.Thaddeus G. Golos (UW: University of Wisconsin-Madison)H-Index: 31
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#1Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
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