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On the Mechanism of Membrane Protein Crystallization in Lipidic Mesophases

Published on Dec 3, 2008in Crystal Growth & Design4.153
· DOI :10.1021/cg800693r
Martin Caffrey56
Estimated H-index: 56
Abstract
The in meso method for crystallizing membrane proteins has been shown to work with a range of different protein types. The method involves reconstituting the target protein into the bilayer of a bicontinuous lipid mesophase followed by an induced phase separation brought on by the addition of a precipitant. A mechanism has been proposed for how in meso crystallogenesis comes about at a molecular level. However, aspects of the hypothesis are not fully developed. These are examined and expanded on here by considering the behavior of related but simpler systems and processes where the mode of action is understood and founded in experiment. By building up from this series of model, well behaved systems a more complete view of what takes place during the in meso crystallogenesis of membrane proteins is obtained. In addition to the underlying theory, some of the more practical aspects of the method are explored and their basis in the hypothesis is established. This treatment suggests a host of experiments to fu...
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Abstract We report on the phase behavior of a technical grade and commercially available diglycerol monoisostearate, C41V, and its use for the preparation of nanostructured liquid crystal dispersions (hexosomes). C41V in water forms a reverse hexagonal liquid crystal at room temperature and in a wide range of concentrations (0.5–95 wt%); this hexagonal liquid crystal is stable up to 70 °C. A simple and effective method has been developed to disperse hexosomes with an encapsulated active molecule...
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X-ray crystallographic methods can be used to visualize macromolecules at high resolution. This provides an understanding of molecular mechanisms and an insight into drug development and rational engineering of enzymes used in the industry. Although conventional synchrotron-based X-ray crystallography remains a powerful tool for understanding molecular function, it has experimental limitations, including radiation damage, cryogenic temperature, and static structural information. Serial femtoseco...
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#1Rika Takeuchi (TUAT: Tokyo University of Agriculture and Technology)H-Index: 1
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#1Jialu Zha (CAS: Chinese Academy of Sciences)
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#1Zina Al-Sahouri (ASU: Arizona State University)H-Index: 1
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#1Pikyee Ma (Trinity College, Dublin)H-Index: 3
#2Dietmar Weichert (Trinity College, Dublin)H-Index: 6
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