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Evaluation of Flinders Technology Associates cards for storage and molecular detection of avian metapneumoviruses.

Published on Mar 4, 2014in Avian Pathology1.96
· DOI :10.1080/03079457.2014.885114
Faez Awad6
Estimated H-index: 6
(University of Liverpool),
Matthew Baylis39
Estimated H-index: 39
(University of Liverpool)
+ 1 AuthorsKannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Cite
Abstract
The feasibility of using Flinders Technology Associates (FTA) cards for the molecular detection of avian metapneumovirus (aMPV) by reverse transcriptase-polymerase chain reaction (RT-PCR) was investigated. Findings showed that no virus isolation was possible from aMPV-inoculated FTA cards, confirming viral inactivation upon contact with the cards. The detection limits of aMPV from the FTA card and tracheal organ culture medium were 101.5 median ciliostatic doses/ml and 100.75 median ciliostatic doses/ml respectively. It was possible to perform molecular characterization of both subtypes A and B aMPV using inoculated FTA cards stored for up to 60 days at 4 to 6°C. Tissues of the turbinate, trachea and lung of aMPV-infected chicks sampled either by direct impression smears or by inoculation of the tissue homogenate supernatants onto the FTA cards were positive by RT-PCR. However, the latter yielded more detections. FTA cards are suitable for collecting and transporting aMPV-positive samples, providing a rel...
  • References (38)
  • Citations (13)
Cite
References38
Newest
Published on Jul 1, 2012in Veterinary Pathology2.12
Saad Gharaibeh11
Estimated H-index: 11
(JUST: Jordan University of Science and Technology),
M. Shamoun1
Estimated H-index: 1
(JUST: Jordan University of Science and Technology)
Avian metapneumovirus (aMPV) is a respiratory virus that infects a range of avian hosts, including chickens and turkeys. Migratory and local wild birds are implicated in aMPV spread among farms, countries, and seasonal outbreaks of the disease. A subtype B aMPV isolate from commercial chicken flocks suffering from respiratory disease was experimentally inoculated oculonasally into 7-week old chickens, young pigeons, and sparrows. Chickens showed minimal tracheal rales, whereas pigeons and sparro...
Published on Jan 1, 2012
Faez Awad6
Estimated H-index: 6
,
Anne Forrester6
Estimated H-index: 6
+ 5 AuthorsJ. Heckmann4
Estimated H-index: 4
Published on Jun 1, 2011in Journal of Virological Methods1.75
E. M. Abdelwhab18
Estimated H-index: 18
(Humboldt University of Berlin),
Dörte Lüschow1
Estimated H-index: 1
(Humboldt University of Berlin)
+ 1 AuthorsHafez M. Hafez24
Estimated H-index: 24
(Humboldt University of Berlin)
Avian influenza viruses (AIVs) infect a wide range of host species including domestic poultry and wild birds; also, AIVs may infect humans in whom some highly pathogenic viruses (HPAIV) may cause acute fatal disease. Accurate laboratory diagnosis of AIV infections requires time-consuming and logistically complex precautionary measures for shipment of specimens or viruses to avoid biohazard exposure. The feasibility was investigated of the Flinders Technology Associates filter paper (FTA® card) f...
Published on Jan 1, 2010in Veterinarski Arhiv0.43
Madaboosi S. Narayanan1
Estimated H-index: 1
(Madras Veterinary College),
Manoharan Parthiban3
Estimated H-index: 3
(Madras Veterinary College)
+ 1 AuthorsK. Kumanan4
Estimated H-index: 4
(Madras Veterinary College)
Published on Dec 1, 2009in Avian Diseases1.31
Aneg L. Cortes9
Estimated H-index: 9
(NCSU: North Carolina State University),
Enrique Montiel7
Estimated H-index: 7
(Merial),
Isabel M. Gimeno18
Estimated H-index: 18
(NCSU: North Carolina State University)
Abstract The use of Flinders Technology Associates (FTA®) filter cards to quantify Marek's disease virus (MDV) DNA for the diagnosis of Marek's disease (MD) and to monitor MD vaccines was evaluated. Samples of blood (43), solid tumors (14), and feather pulp (FP; 36) collected fresh and in FTA cards were analyzed. MDV DNA load was quantified by real-time PCR. Threshold cycle (Ct) ratios were calculated for each sample by dividing the Ct value of the internal control gene (glyceraldehyde-3-phospha...
Published on Feb 1, 2008in Avian Pathology1.96
Ye Htut Aung3
Estimated H-index: 3
,
Martin Liman4
Estimated H-index: 4
+ 1 AuthorsSilke Rautenschlein20
Estimated H-index: 20
Swollen head syndrome (SHS) associated with avian metapneumovirus (aMPV) subtype A or subtype B in broilers and broiler breeders has been reported worldwide. Data about pathogenesis of aMPV subtypes A and B in broilers are scarce. It has been difficult to reproduce swollen sinuses in chickens with aMPV under experimental conditions. In the field, SHS in broilers is suspected to be induced by combined infections with different respiratory pathogens. The objectives of the present study were to com...
Published on Mar 1, 2007in Avian Diseases1.31
Hugo Moscoso5
Estimated H-index: 5
(UGA: University of Georgia),
Jose J. Bruzual1
Estimated H-index: 1
(UGA: University of Georgia)
+ 1 AuthorsCharles L. Hofacre29
Estimated H-index: 29
(UGA: University of Georgia)
Abstract The feasibility of using liver impressions on Flinders Technology Associates (FTA®) filter paper for the collection, inactivation, and molecular analysis of fowl adenovirus (FAV) was evaluated. FAV I European Union (EU) serotype 1 spotted on FTA® was shown to be inactivated using specific-pathogen-free (SPF) primary chicken embryo liver cell culture as indicated by absence of cytopathic effect. Sensitivity of the polymerase chain reaction (PCR) test using tenfold dilutions of allantoic ...
Published on Feb 1, 2007in Veterinary Immunology and Immunopathology1.85
Martin Liman4
Estimated H-index: 4
,
Silke Rautenschlein20
Estimated H-index: 20
Abstract Most of the studies regarding the immunopathogenesis of avian Metapneumovirus (aMPV) have been done with subtype C of aMPV. Not much is known about the immunopathogenesis of aMPV subtypes A and B in turkeys. Specifically, local immune reactions have not been investigated yet. We conducted two experiments in commercial turkeys. We investigated local and systemic humoral and cell mediated immune reactions following infection with an attenuated vaccine strain of aMPV subtype B (Experiment ...
Published on Dec 1, 2006in Journal of Virological Methods1.75
Linda B. Purvis7
Estimated H-index: 7
(UGA: University of Georgia),
Pedro Villegas23
Estimated H-index: 23
(UGA: University of Georgia),
Francisco Perozo10
Estimated H-index: 10
(UGA: University of Georgia)
Infectious bursal disease virus (IBDV) is an important poultry pathogen and is distributed world wide that can cause immune suppression and lesions of the bursa of Fabricius. The main component of the virus, VP2, is not only responsible for the bird's immune response, but is important for the molecular identification of this virus as well. The nucleic acid of the virus must be adequately preserved to be analyzed by reverse-transcriptase PCR (RT-PCR) and sequenced for the molecular characterizati...
Published on Sep 1, 2006in Avian Diseases1.31
Hugo Moscoso5
Estimated H-index: 5
(UGA: University of Georgia),
I. R. Alvarado8
Estimated H-index: 8
(UGA: University of Georgia),
Charles L. Hofacre29
Estimated H-index: 29
(UGA: University of Georgia)
Abstract We investigated the feasibility of using FTA® filter cards for the storage of bursas of Fabricius containing infectious bursal disease virus (IBDV) and for IBDV detection by reverse transcriptase (RT)–polymerase chain reaction (PCR), and characterization by restriction fragment length polymorphism (RFLP) or nucleotide sequencing. The FTA® card is a cotton-based cellulose membrane containing lyophilized chemicals that lyses many types of bacteria and viruses. IBDV was inactivated upon co...
Cited By13
Newest
Published on Sep 1, 2019in Preventive Veterinary Medicine2.30
Jaime A. Cardona-Ospina1
Estimated H-index: 1
(Grupo México),
Manuel F. Villalba-Miranda (Grupo México)+ 2 AuthorsJuan Carlos Sepúlveda-Arias6
Estimated H-index: 6
(Grupo México)
Abstract Background Detection and characterization of viral RNA pathogens from fieldwork are challenging due to the instability of the RNA molecule. FTA cards® have proved useful for sample storage and latter identification of pathogens with importance for agricultural, animal and human health: however, for optimal handling, processing, and biosafety measures are not well-established. Objective This systematic review aims to summarize the reported effectiveness of FTA cards® for storage and tran...
Camila Neves de Almeida3
Estimated H-index: 3
(UFRGS: Universidade Federal do Rio Grande do Sul),
Thales Quedi Furian6
Estimated H-index: 6
(UFRGS: Universidade Federal do Rio Grande do Sul)
+ 6 AuthorsHamilton Luiz de Souza Moraes10
Estimated H-index: 10
(UFRGS: Universidade Federal do Rio Grande do Sul)
Published on Jul 4, 2018in Avian Pathology1.96
Basim Manswr1
Estimated H-index: 1
(University of Liverpool),
Christopher Ball2
Estimated H-index: 2
(University of Liverpool)
+ 2 AuthorsKannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
ABSTRACTSequence variability in the S1 gene determines the genotype of infectious bronchitis virus (IBV) strains. A single RT-PCR assay was developed to amplify and sequence the full S1 gene for six classical and variant IBVs (M41, D274, 793B, IS/885/00, IS/1494/06 and Q1) enriched in allantoic fluid (AF) or the same AF inoculated onto Flinders Technology Association (FTA) cards. Representative strains from each genotype were grown in specific-pathogen-free eggs and RNA was extracted from AF. Fu...
Published on May 4, 2018in Avian Pathology1.96
Guillaume Croville6
Estimated H-index: 6
(University of Toulouse),
Charlotte Foret3
Estimated H-index: 3
(University of Toulouse)
+ 6 AuthorsJean-Luc Guérin16
Estimated H-index: 16
(University of Toulouse)
ABSTRACTRespiratory syndromes (RS) are among the most significant pathological conditions in edible birds and are caused by complex coactions of pathogens and environmental factors. In poultry, low pathogenic avian influenza A viruses, metapneumoviruses, infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma spp. Escherichia coli and/or Ornithobacterium rhinotracheale in turkeys are considered as key co-infectious agents of RS. Aspergillus sp., Pasteurella multocida, Avibact...
Published on Nov 21, 2017in PLOS Biology
Yiping Zou1
Estimated H-index: 1
(UQ: University of Queensland),
Michael G. Mason15
Estimated H-index: 15
(UQ: University of Queensland)
+ 5 AuthorsJosé Ramón Botella34
Estimated H-index: 34
(UQ: University of Queensland)
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washi...
Published on May 19, 2017in Critical Reviews in Biotechnology7.05
Rui Hua Tang1
Estimated H-index: 1
(Xi'an Jiaotong University),
Hui Yang13
Estimated H-index: 13
(NPU: Northwestern Polytechnical University)
+ 7 AuthorsFeng Xu49
Estimated H-index: 49
(Xi'an Jiaotong University)
AbstractIn recent years, paper-based point-of-care testing (POCT) has been widely used in medical diagnostics, food safety and environmental monitoring. However, a high-cost, time-consuming and equipment-dependent sample pretreatment technique is generally required for raw sample processing, which are impractical for low-resource and disease-endemic areas. Therefore, there is an escalating demand for a cost-effective, simple and portable pretreatment technique, to be coupled with the commonly us...
Published on May 4, 2017in Avian Pathology1.96
Christopher Ball2
Estimated H-index: 2
(University of Liverpool),
Faez Awad6
Estimated H-index: 6
(University of Liverpool)
+ 3 AuthorsKannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
ABSTRACTAn investigation was undertaken of the extent of genetic variation occurring within infectious bronchitis virus (IBV) vaccine strains following vaccination of day-old broiler chicks. Chicks were divided into seven groups, with two groups receiving single Massachusetts (Mass) vaccinations while the other four were inoculated with combinations of different IBV serotypes; Mass, 793B, D274 and Arkansas (Ark). The remaining group was maintained as an unvaccinated control. Following vaccinatio...
Published on Dec 1, 2016in Journal of Clinical Microbiology4.96
C.E. Scantlebury7
Estimated H-index: 7
(University of Liverpool),
G. L. Pinchbeck32
Estimated H-index: 32
(University of Liverpool)
+ 7 AuthorsAlan J. McCarthy31
Estimated H-index: 31
(University of Liverpool)
ABSTRACT Histoplasma capsulatum var. farciminosum, the causative agent of epizootic lymphangitis (EZL), is endemic in parts of Africa. Diagnosis based on clinical signs and microscopy lacks specificity and is a barrier to further understanding this neglected disease. Here, a nested PCR method targeting the internal transcribed spacer (ITS) region of the rRNA operon was validated for application to equine clinical samples. Twenty-nine horses with signs of EZL from different climatic regions of Et...
Published on Apr 1, 2016in Research in Veterinary Science1.75
Faez Awad6
Estimated H-index: 6
(University of Liverpool),
Rajesh Chhabra5
Estimated H-index: 5
(University of Liverpool)
+ 5 AuthorsKannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Pathogenesis of an IS/885/00-like (IS/885) strain of variant infectious bronchitis virus (IBV) was examined in one day old specific pathogen free (SPF) and commercial broiler chicks. Chicks were humanely euthanized at 3, 6, 9, 12, 15, 21 and 28 days post infection (dpi) for necropsy examination, and tissues were collected for histopathology and virus detection by reverse transcription polymerase chain reaction (RT-PCR). Respiratory clinical signs and gross lesions consisting of tracheal caseous ...
Published on Mar 3, 2016in Avian Pathology1.96
Faez Awad6
Estimated H-index: 6
,
Sally Hutton2
Estimated H-index: 2
+ 2 AuthorsKannan Ganapathy12
Estimated H-index: 12
ABSTRACTGroups of one-day-old broiler chicks were vaccinated via the oculo-nasal route with different live infectious bronchitis virus (IBV) vaccines: Massachusetts (Mass), 793B, D274 or Arkansas (Ark). Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at intervals and their tracheas collected for ciliary activity assessment and for the detection of CD4+, CD8+ and IgA-bearing B cells by immunohistochemistry (IHC). Blood samples were collected at in...
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