Monitoring crystallization experiments using dynamic light scattering: Assaying and monitoring protein crystallization in solution

Various analytical techniques may be valuable as precrystallization assays for monitoring and screening various protein crystal growth conditions. Dynamic light scattering (DLS) offers unique capabilities for such studies. DLS provides a measure of the translational diffusion coefficient, D, of the protein molecules. Assuming a spherical model for the protein, its hydrodynamic diameter, d, can then be estimated. DLS can be used to monitor...