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Regulatory role of Toll-like receptor 2 during infection with Trypanosoma cruzi

Published on Dec 1, 2004in Journal of Endotoxin Research
· DOI :10.1177/09680519040100060801
Catherine Ropert21
Estimated H-index: 21
(FIOCRUZ: Oswaldo Cruz Foundation),
Ricardo T. Gazzinelli71
Estimated H-index: 71
(UFMG: Universidade Federal de Minas Gerais)
Abstract
Previous studies have emphasized the role of Toll-like receptors (TLRs) and myeloid differentiation factor 88 (MyD88) during infection with protozoan parasites. TLR2 was shown to be important for induction of cytokine synthesis by macrophages exposed to the purified glycosylphosphatidylinositol (GPI)-anchored mucin-like glycoproteins of Trypanosoma cruzi trypomastigotes (tGPIm). On the other hand, MyD88 —/— mice, but not TLR2 —/— mice, showed impaired cytokine production and resistance to infection with T. cruzi parasites. Here we evaluate the importance of MyD88 and TLR2 in MAPK activation and cytokine synthesis by macrophages exposed to live T. cruzi parasites and compared to tGPIm. The absence of MAPK phosphorylation in TLR2- and MyD88-deficient macrophages exposed to tGPIm correlated with the incapacity to induce cytokine release in these cells. In contrast, activation of MAPK and synthesis of pro-inflammatory cytokines were not abrogated in TLR2-deficient macrophages exposed to live T. cruzi parasite...
  • References (18)
  • Citations (59)
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Studies performed in vitro suggest that activation of Toll-like receptors (TLRs) by parasite-derived molecules may initiate inflammatory responses and host innate defense mechanisms against Trypanosoma cruzi . Here, we evaluated the impact of TLR2 and myeloid differentiation factor 88 (MyD88) deficiencies in host resistance to infection with T. cruzi. Our results show that macrophages derived from TLR2 −/− and MyD88 −/− mice are less responsive to GPI-mucin derived from T. cruzi trypomastigotes ...
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