Original paper
A procedure for setting up high-throughput nanolitre crystallization experiments. I. Protocol design and validation
Abstract
A protocol for setting up nanolitre sitting-drop vapour-diffusion experiments is reported. The procedure uses standard crystallization screening kits and 96-well crystallization plates. Reservoir solutions are transferred from 96-deep-well blocks to crystallization plates in a single step with a Robbins-Hydra pipettor. Nanolitre droplets of protein as well as reservoir solution are dispensed by a Cartesian pipetting instrument. Experiments have...
Paper Details
Title
A procedure for setting up high-throughput nanolitre crystallization experiments. I. Protocol design and validation
Published Date
Mar 15, 2003
Volume
36
Issue
2
Pages
308 - 314
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History