A Cell-Free Protein Synthesis System from Insect Cells
Abstract
The Transdirect insect cell is a newly developed in vitro translation system for mRNA templates, which utilizes an extract from cultured Spodoptera frugiperda 21 (Sf21) insect cells. An expression vector, pTD1, which includes a 5′-untranslated region (UTR) sequence from a baculovirus polyhedrin gene as a translational enhancer, was also developed to obtain maximum performance from the insect cell-free protein synthesis system. This combination...
Paper Details
Title
A Cell-Free Protein Synthesis System from Insect Cells
Published Date
Nov 6, 2009
Journal
Pages
31 - 42
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