Intein-mediated one-step purification of Escherichia coli secreted human antibody fragments
Abstract
In this work, we apply self-cleaving affinity tag technology to several target proteins secreted into the Escherichia coli periplasm, including two with disulfide bonds. The target proteins were genetically fused to a self-cleaving chitin-binding domain-intein tag for purification via a chitin-agarose affinity resin. By attaching the intein-tagged fusion genes to the PelB secretion leader sequence, the tagged target proteins were secreted to the...
Paper Details
Title
Intein-mediated one-step purification of Escherichia coli secreted human antibody fragments
Published Date
Apr 1, 2011
Volume
76
Issue
2
Pages
221 - 228
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