Monovalent Metal Ions Play an Essential Role in Catalysis and Intersubunit Communication in the Tryptophan Synthase Bienzyme Complex
Abstract
This investigation shows that the alpha 2 beta 2 tryptophan synthase bienzyme complex from Salmonella typhimurium is subject to monovalent metal ion activation. The effects of the monovalent metal ions Na+ and K+ were investigated using rapid scanning stopped-flow (RSSF), single-wavelength stopped-flow (SWSF), and steady-state techniques. RSSF measurements of individual steps in the reaction of L-serine and indole to give L-trytophan (the...
Paper Details
Title
Monovalent Metal Ions Play an Essential Role in Catalysis and Intersubunit Communication in the Tryptophan Synthase Bienzyme Complex
Published Date
Jul 25, 1995
Journal
Volume
34
Issue
29
Pages
9466 - 9476
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