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Characterization of Israeli avian metapneumovirus strains in turkeys and chickens

Published on Jun 1, 2005in Avian Pathology1.96
· DOI :10.1080/03079450500112625
Caroline Banet-Noach14
Estimated H-index: 14
,
Lubov Simanov9
Estimated H-index: 9
,
Shimon Perk9
Estimated H-index: 9
Cite
Abstract
Although the disease associated with the avian metapneumovirus (aMPV) has been described in Israel for more than 20 years and one Israeli isolate was characterized as subtype A, this is the first study investigating the strains circulating in Israel (period 2002 to 2004). Viral RNA was detected by reverse transcription-polymerase chain reaction in 44% of the examined flocks, and one-half of the virus isolation attempts were successful. Characterization showed that 34 non-vaccinated turkey flocks had aMPV subtype B, three had aMPV subtype A and three remained undetermined. Sequence analysis of part of the attachment protein G gene (1161 base pairs) showed a relative homogeneity within the Israeli subtype B group, although they were different from the European and vaccine strains. Seven out of 10 vaccinated flocks were reverse transcription-polymerase chain reaction-positive for aMPV, and sequence analysis of 239 base pairs of the G gene revealed that field strains and not vaccine strains were recovered fro...
  • References (32)
  • Citations (29)
Cite
References32
Newest
Published on Apr 1, 2004in Clinical Microbiology Reviews17.75
Andrew J. Easton1
Estimated H-index: 1
(Coventry Health Care),
Joseph B. Domachowske2
Estimated H-index: 2
(SU: Syracuse University),
Helene F. Rosenberg50
Estimated H-index: 50
(NIH: National Institutes of Health)
Pneumoviruses are single-stranded, negative-sense, nonsegmented RNA viruses of the family Paramyxoviridae, subfamily Pneumovirinae, and include pathogens that infect humans (respiratory syncytial virus and human metapneumovirus), domestic mammals (bovine, ovine, and caprine respiratory syncytial viruses), rodents (pneumonia virus of mice), and birds (avian metapneumovirus). Among the topics considered in this review are recent studies focused on the roles of the individual virus-encoded componen...
Published on Feb 1, 2004in Avian Pathology1.96
Michael Hess33
Estimated H-index: 33
,
M.B. Huggins2
Estimated H-index: 2
+ 1 AuthorsU. Heincz2
Estimated H-index: 2
Vaccinated and non-vaccinated specified pathogen-free White Leghorn laying chickens were challenged at peak of lay by the intravenous or oculonasal route with a virulent avian metapneumovirus (aMPV) subtype B chicken strain. Severe clinical signs and a drop in egg production were induced in the non-vaccinated intravenously challenged birds whereas the vaccinates were not affected. Live virus excretion was demonstrated in the faeces and respiratory tract of non-vaccinated hens for up to 7 days po...
Published on Oct 1, 2003in Avian Pathology1.96
Caroline Banet-Noach14
Estimated H-index: 14
,
Lubov Simanov9
Estimated H-index: 9
,
Mertyn Malkinson4
Estimated H-index: 4
During a recent epizootic, losses due to West Nile virus (WNV) infection in young goose flocks were estimated to be far greater than expected if mosquito-borne transmission was the principal route of infection. Contact transmission was investigated experimentally as an alternative explanation. A group of 10, 3-week-old geesewere inoculated subcutaneously and placed in one insect-proof room with 20 geese of the same age. A group of10 geese were housed in an adjacent insect-proof room to serve as ...
Published on Apr 1, 2003in Journal of Clinical Microbiology4.96
Rene Alvarez3
Estimated H-index: 3
(USDA: United States Department of Agriculture),
Humphrey C. M. Lwamba6
Estimated H-index: 6
(UMN: University of Minnesota)
+ 2 AuthorsBruce S. Seal37
Estimated H-index: 37
(USDA: United States Department of Agriculture)
A serologically distinct avian metapneumovirus (aMPV) was isolated in the United States after an outbreak of turkey rhinotracheitis (TRT) in February 1997. The newly recognized U.S. virus was subsequently demonstrated to be genetically distinct from European subtypes and was designated aMPV serotype C (aMPV/C). We have determined the nucleotide sequence of the gene encoding the cell attachment glycoprotein (G) of aMPV/C (Colorado strain and three Minnesota isolates) and predicted amino acid sequ...
Published on Feb 1, 2003in Virus Research2.74
M. Kariuki Njenga18
Estimated H-index: 18
(UMN: University of Minnesota),
Humphrey C. M. Lwamba6
Estimated H-index: 6
(UMN: University of Minnesota),
Bruce S. Seal37
Estimated H-index: 37
(ARS: Agricultural Research Service)
Abstract Avian pneumovirus (APV, Turkey rhinotracheitis virus ) and Human metapneumovirus (hMPV) are pathogens of birds and humans, respectively, that are associated with upper respiratory tract infections. Based on their different genomic organization and low level of nucleotide (nt) and amino acid (aa) identity with paramyxoviruses in the genus Pneumovirus , APV and hMPV have been classified into a new genus referred to as Metapneumovirus . First isolated in 1970s, APV strains have since been ...
Published on Jul 1, 2002in Avian Diseases1.31
A. N. Alkhalaf1
Estimated H-index: 1
(Ohio Agricultural Research and Development Center),
L. A. Ward9
Estimated H-index: 9
(Ohio Agricultural Research and Development Center)
+ 1 AuthorsY. M. Saif29
Estimated H-index: 29
(Ohio Agricultural Research and Development Center)
SUMMARY. The pathogenicity, transmissibility, tissue distribution, and persistence of avian pneumovirus (APV) in turkey poults were investigated in three experiments. In the first experiment, we inoculated 2-wk-old commercial turkey poults oculonasally with APV alone or in combination with Bordetella avium. In the dually infected group, clinical signs were more severe, the virus persisted longer, the bacteria invaded more respiratory tissues, and the birds had higher antibody titer than the grou...
Published on May 1, 2002in Veterinary Pathology2.12
Faris F. Jirjis7
Estimated H-index: 7
(UMN: University of Minnesota),
Sally Noll17
Estimated H-index: 17
+ 2 AuthorsDaniel P. Shaw17
Estimated H-index: 17
(UMN: University of Minnesota)
Avian pneumovirus (APV) is the cause of a respiratory disease of turkeys characterized by coughing, ocular and nasal discharge, and swelling of the infraorbital sinuses. Sixty turkey poults were reared in isolation conditions. At 3 weeks of age, serum samples were collected and determined to be free of antibodies against APV, avian influenza, hemorrhagic enteritis, Newcastle disease, Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, Ornithobacterium rhinotracheale, and Borde...
Published on Apr 1, 2002in Avian Pathology1.96
Jane K. A. Cook31
Estimated H-index: 31
,
Dave Cavanagh18
Estimated H-index: 18
(Newbury College)
The available detection methods for avian pneumoviruses (turkey rhinotracheitis virus; genus Metapneumovirus ) in turkeys, domestic fowl and other species are reviewed. The advantages and disadvantages of virus isolation techniques, virus or genome (polymerase chain reaction) detection and serology are discussed. Some of the problems likely to be encountered are considered, including the detection of yet to be discovered subtypes, as are the factors that are likely to influence the outcome of th...
Published on Jun 1, 2001in Nature Medicine30.64
Bernadette G. van den Hoogen27
Estimated H-index: 27
(EUR: Erasmus University Rotterdam),
Jan C. de Jong15
Estimated H-index: 15
(EUR: Erasmus University Rotterdam)
+ 4 AuthorsAlbert D. M. E. Osterhaus112
Estimated H-index: 112
(EUR: Erasmus University Rotterdam)
From 28 young children in the Netherlands, we isolated a paramyxovirus that was identified as a tentative new member of the Metapneumovirus genus based on virological data, sequence homology and gene constellation. Previously, avian pneumovirus was the sole member of this recently assigned genus, hence the provisional name for the newly discovered virus: human metapneumovirus. The clinical symptoms of the children from whom the virus was isolated were similar to those caused by human respiratory...
Published on Nov 1, 2000in Journal of General Virology2.81
M. H. Bäyon-Auboyer5
Estimated H-index: 5
,
Claire Arnauld3
Estimated H-index: 3
+ 1 AuthorsN. Eterradossi23
Estimated H-index: 23
Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99·7% nt and 99·0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70·0–80·5% and 77·6–97·2%, respectively, with the L gene sharing 76·1% nt and 85·3% aa identity with that of a...
Cited By29
Newest
Published on Nov 2, 2015in Avian Pathology1.96
Sandra Hartmann2
Estimated H-index: 2
,
Hicham Sid3
Estimated H-index: 3
,
Silke Rautenschlein20
Estimated H-index: 20
Avian metapneumovirus (aMPV) is a pathogen with worldwide distribution, which can cause high economic losses in infected poultry. aMPV mainly causes infection of the upper respiratory tract in both chickens and turkeys, although turkeys seem to be more susceptible. Little is known about virus–host interactions at epithelial surfaces after aMPV infection. Tracheal organ cultures (TOC) are a suitable model to investigate virus–host interaction in the respiratory epithelium. Therefore, we investiga...
Published on Oct 1, 2015in Archives of Virology2.26
Bingling Yun6
Estimated H-index: 6
,
Yanni Gao6
Estimated H-index: 6
+ 9 AuthorsYanping Zhang6
Estimated H-index: 6
The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD50 assay developed in this study. Interes...
Published on Apr 1, 2015in Research in Veterinary Science1.75
Thunai Al-Shekaili1
Estimated H-index: 1
(University of Liverpool),
Matthew Baylis39
Estimated H-index: 39
(University of Liverpool),
Kannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Abstract Infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) are economically important viral pathogens infecting chickens globally. Identification of endemic IBV and aMPV strains promotes better control of both diseases and prevents production losses. Orophrayngeal swab samples were taken from 2317 birds within 243 different backyard flocks in Oman. Swabs from each flock were examined by RT-PCR using part-S1 and G gene primers for IBV and aMPV respectively. Thirty-nine chicken fl...
Published on Jun 1, 2013in Avian Pathology1.96
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 4 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
In recent years, special attention has been paid to real-time polymerase chain reaction (PCR) for avian metapneumovirus (AMPV) diagnosis, due to its numerous advantages over classical PCR. A new multiplex quantitative real-time reverse transcription-PCR (qRT-PCR) with molecular beacon probe assay, designed to target the SH gene, was developed. The test was evaluated in terms of specificity, sensitivity and repeatability, and compared with conventional RT nested-PCR based on the G gene. All of th...
Published on Jan 1, 2013in Virology Journal2.46
Ra Cha2
Estimated H-index: 2
(USDA: United States Department of Agriculture),
Qingzhong Yu13
Estimated H-index: 13
(USDA: United States Department of Agriculture),
L. Zsak28
Estimated H-index: 28
(USDA: United States Department of Agriculture)
Background Avian metapneumovirus subtype C (aMPV/C) causes severe upper respiratory disease in turkeys. Previous report revealed the presence of aMPV/C in wild birds in the southeast regions of the U.S.
Published on Dec 1, 2012in Pesquisa Veterinaria Brasileira0.30
Márcia Bianchi dos Santos3
Estimated H-index: 3
(State University of Campinas),
Matheus C. Martini4
Estimated H-index: 4
(State University of Campinas)
+ 4 AuthorsClarice Weis Arns16
Estimated H-index: 16
(State University of Campinas)
Avian metapneumovirus (aMPV) is a respiratory pathogen associated with the swollen head syndrome (SHS) in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B) are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes) of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protect...
Published on Oct 1, 2011in Avian Pathology1.96
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna),
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
+ 2 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
Avian metapneumovirus (aMPV) subtype A was isolated from 7-week-old turkeys showing respiratory disease typical of turkey rhinotracheitis. Comparison of the virus sequence with previously determined vaccine marker sequences showed that the virulent virus had originated from a licensed live subtype A aMPV vaccine. The vaccine had neither been in use on the farm within a period of at least 6 months nor had it been used on farms within a distance of approximately 5 km. Isolation of the virus and ex...
Published on Oct 1, 2011in Avian Pathology1.96
P. A. N. Felippe7
Estimated H-index: 7
(State University of Campinas),
Luciana Helena Antoniassi da Silva3
Estimated H-index: 3
(State University of Campinas)
+ 2 AuthorsClarice Weis Arns16
Estimated H-index: 16
(State University of Campinas)
The aim of the present study was to determine whether avian metapneumovirus (aMPV)-related viruses were present in wild and synanthropic birds in Brazil. Therefore, we analysed samples from wild birds, feral pigeons and domestic chickens in order to perform a phylogenetic comparison. To detect the presence of aMPV, a nested reverse transcriptase-polymerase chain reaction was performed with the aim of amplifying a fragment of 270 bases for subtype A and 330 bases for subtype B, comprising the gen...
Published on Jul 1, 2011in Journal of Virology4.32
Yosef Sabo4
Estimated H-index: 4
(TAU: Tel Aviv University),
Marcelo Ehrlich26
Estimated H-index: 26
(TAU: Tel Aviv University),
Eran Bacharach22
Estimated H-index: 22
(TAU: Tel Aviv University)
ABSTRACT YXXL motifs in cellular and viral proteins have a variety of functions. The matrix (M) protein of the respiratory pathogen human metapneumovirus (hMPV) contains two such conserved motifs—YSKL and YAGL. We mutated these sequences to analyze their contributions to hMPV infectivity. The mutant clones were capable of intracellular replication; however, the YAGL but not YSKL mutants were defective at spreading in infected cultures. We improved the reverse genetics system for hMPV and generat...
Published on Jun 1, 2011in Virus Genes1.62
Qingzhong Yu3
Estimated H-index: 3
(ARS: Agricultural Research Service),
Carlos N. Estevez2
Estimated H-index: 2
(ARS: Agricultural Research Service)
+ 2 AuthorsLaszlo Zsak5
Estimated H-index: 5
(ARS: Agricultural Research Service)
The second matrix (M2) gene of avian metapneumovirus subgroup C (aMPV-C) contains two overlapping open reading frames (ORFs), encoding two putative proteins, M2-1 and M2-2. Both proteins are believed to be involved in viral RNA transcription or replication. To further characterize the function of the M2-2 protein in virus replication, the non-overlapping region of the M2-2 ORF was deleted from an infectious cDNA clone of the aMPV-C strain, and a viable virus was rescued by using reverse genetics...
View next paperNucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup