Study of Ribonuclease precrystallization by fluorescence anisotropy

The purpose of this work is to study protein interactions under crystallization conditions. Fluorescence appears a suitable method to follow the prenucleation phase since it is sensitive to any factor affecting the rate of rotational diffusion. We present a study using bovine pancreatic Ribonuclease A. The fluorescent signal is obtained from a label covalently attached to one of the histidines of the active site, the fluorescence anisotropy of...