Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli
Abstract
Amelogenin is a dental enamel matrix protein involved in formation of dental enamel. In this study, we have expressed two different recombinant murine amelogenins in Escherichia coli: the untagged rM179, and the histidine tagged rp(H)M180, identical to rM179 except that it carries the additional N-terminal sequence MRGSHHHHHHGS. The effects of the histidine tag on expression levels, and on growth properties of the amelogenin expressing cells...
Paper Details
Title
Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli
Published Date
Jul 1, 2006
Volume
48
Issue
1
Pages
134 - 141
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