Linguistics

Philosophy

Neuroscience

Biology

Amylase

Finger millet

Inhibitory postsynaptic potential

Biochemistry

Botany

Food science

Paleontology

Weed

Agronomy

Enzyme

Eleusine indica

Chemistry

Coat

Composition (language)

Eleusine

Food Chemistry

Inhibitors of alpha glucosidase and pancreatic amylase play a vital role in the clinical management of postprandial hyperglycemia. Although, powerful synthetic inhibitors are available, natural inhibitors are potentially safer. Phenolic compounds from the millet seed coat were extracted with acidified methanol and characterised by HPLC and ESI-MS. These phenolics showed strong inhibition towards α-glucosidase and pancreatic amylase and the IC₅₀ values were 16.9 and 23.5μg of phenolics, respectively. The enzyme kinetic studies, using Michaelis-Menton and Lineweaver-Burk equations, indicated that, in the presence of millet phenolics, the Michaelis-Menton constant (K m) remained constant but the maximal velocity (V max) decreased, revealing a non-competitive type of inhibition. The study indicated the therapeutic potentiality of millet phenolics in the management of postprandial hyperglycemia.

Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of α-glucosidase and pancreatic amylase