Effective oligonucleotide-mediated gene disruption in ES cells lacking the mismatch repair protein MSH3

Volume: 13, Issue: 8, Pages: 686 - 694
Published: Jan 28, 2006
Abstract
We have previously demonstrated that site-specific insertion, deletion or substitution of one or two nucleotides in mouse embryonic stem cells (ES cells) by single-stranded deoxyribo-oligonucleotides is several hundred-fold suppressed by DNA mismatch repair (MMR) activity. Here, we have investigated whether compound mismatches and larger insertions escape detection by the MMR machinery and can be effectively introduced in MMR-proficient cells....
Paper Details
Title
Effective oligonucleotide-mediated gene disruption in ES cells lacking the mismatch repair protein MSH3
Published Date
Jan 28, 2006
Volume
13
Issue
8
Pages
686 - 694
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