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Studies of crystal growth mechanisms of proteins by electron microscopy.

Published on Apr 1, 1990in Journal of Molecular Biology5.07
· DOI :10.1016/0022-2836(90)90235-E
S.D. Durbin1
Estimated H-index: 1
(UCSD: University of California, San Diego),
G. Feher49
Estimated H-index: 49
(UCSD: University of California, San Diego)
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Abstract
Abstract We have used electron microscopy to examine the surfaces of lysozyme crystals and deduce mechanisms of crystal growth. We find that growth occurs by a lattice defect mechanism at low supersaturation and by two-dimensional nucleation at high supersaturation. Step velocities and two-dimensional nucleation rates are obtained, and their dependence on supersaturation is compared with theory. Some features of the observed surface structure can be related to the specific topology and strengths of the bonds in the P 4 3 2 1 2 lattice. Preliminary results on the early stages of nucleation and the phenomenon of cessation of growth are presented.
  • References (13)
  • Citations (91)
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References13
Newest
Published on Mar 14, 2007
John D. Weeks36
Estimated H-index: 36
(Bell Labs),
George H. Gilmer50
Estimated H-index: 50
(Bell Labs)
Published on Aug 1, 1986in Journal of Crystal Growth1.57
S.D. Durbin2
Estimated H-index: 2
(UCSD: University of California, San Diego),
G. Feher3
Estimated H-index: 3
(UCSD: University of California, San Diego)
Abstract The protein concentration dependence of the growth rate of lysozyme crystals (tetragonal form) was investigated at two NaCl concentrations (3.5%, 5% w/v). The rates for different crystallographic faces were found to have different concentration dependences, resulting in a changing crystal shape with concentration. The experimental results were compared with standard crystal growth theories. The strong concentration dependence could be fitted at high supersaturation by a two-dimensional ...
Published on Apr 1, 1986in Physical Review B3.74
van Saarloos W14
Estimated H-index: 14
(Bell Labs),
George H. Gilmer50
Estimated H-index: 50
(Bell Labs)
Motivated by advances in laser scattering experiments on crystal-melt interfaces during growth conditions, we discuss the interface fluctuations of a faceted crystal during nucleation-dominated growth. If the nucleation rate is large enough so that each layer grows as a result of multiple nucleation events, the properties of long-wavelength interface fluctuations can be understood in terms of a simple random-walk picture. Accordingly, apart from a change in length scale, the interface fluctuatio...
Published on Jan 1, 1986in Journal of Crystal Growth1.57
K.W. Keller1
Estimated H-index: 1
Abstract After previous evaporation, growth experiments have been carried out by vapour deposition of NaCl onto NaCl under the following experimental conditions; temperature T =100–350°C, evaporation rate R =0.002–2 nm s -1 , and evaporated layer thickness A =0.04–200 nm. Electron microscope observations of step patterns using the gold decoration technique have revealed the great variety of growth modes: random two-dimensional nucleation, hill formation by the spiral mechanism and by repeated tw...
Published on Jan 1, 1985in Ultramicroscopy2.64
Luis Bachmann16
Estimated H-index: 16
(TUM: Technische Universität München),
R. Becker1
Estimated H-index: 1
(TUM: Technische Universität München)
+ 3 AuthorsWolfgang Baumeister93
Estimated H-index: 93
Abstract Aqueous suspensions of catalase crystals were freeze-cleaved, deep-etched and either shadowed with Ta/W at 45° or decorated with 0.1–0.9 nm thick deposits of Au and Pt at normal incidence. The electron micrographs of the decorated specimens were processed by correlation averaging and compared with a relief reconstruction obtained from shadowed specimens. Pronounced decoration was observed on the catalase crystals at temperatures between 130 and 180 K. Disregarding the difficulties in in...
Published on Jul 1, 1980in Journal of Crystal Growth1.57
George H. Gilmer50
Estimated H-index: 50
(Bell Labs)
Abstract Transient responses of crystals to the application of a driving force are calculated for three mechanisms of growth. Two-dimensional nucleation is considered in the greatest detail. The transient that results from the nucleation of disk-shaped clusters on a multi-level surface is calcualted by analytical methods. The accuracy of the approximations employed in these methods is assessed by comparison with Monte Carlo results for precisely the same model. Atomistic effects are evaluated us...
Published on Aug 1, 1978in Journal of Molecular Biology5.07
Zvi Kam41
Estimated H-index: 41
(UCSD: University of California, San Diego),
H.B. Shore1
Estimated H-index: 1
(SDSU: San Diego State University),
G. Feher49
Estimated H-index: 49
(UCSD: University of California, San Diego)
Abstract We report on theoretical and experimental work aimed at a systematic approach to the crystallization of proteins. Successful crystallization depends on the competition between the growth rates for compact three-dimensional structures and long-chain structures leading to an amorphous precipitate. Quasi-elastic light scattering was used to monitor the size and shape distribution of small aggregates in a model system (lysozyme) during the pre-nucleation stage. With the aid of a simple mode...
Published on Jan 1, 1976in Journal of Molecular Biology5.07
John Moult8
Estimated H-index: 8
(Weizmann Institute of Science),
Ada Yonath45
Estimated H-index: 45
(Weizmann Institute of Science)
+ 4 AuthorsA. Saya1
Estimated H-index: 1
(Weizmann Institute of Science)
The gross similarity of the conformation of the hen egg-white lysozyme molecule in the triclinic and tetragonal crystal forms is known from an earlier study. In this work we have established the detailed conformation of the molecule in the triclinic form and compared the two structures using appropriately weighted difference maps. An independent model of the triclinic structure has been obtained by use of the real-space refinement technique. There are appreciable conformational differences of ma...
Published on Sep 15, 1975in Journal of Molecular Biology5.07
Stephen C. Harrison107
Estimated H-index: 107
(Harvard University),
Anthony Abraham Jack6
Estimated H-index: 6
(Harvard University)
The three-dimensional structure of tomato bushy stunt virus has been determined by X-ray crystallographic analysis to 16 A resolution, using methods of multiple isomorphous replacement, together with methods based on non-crystallographic symmetry. The relative orientation of the three quasi-equivalent protein subunits is clearly defined in the map. The geometry of dimer contacts appears to be very similar at strict and local dyads, whereas departures from equivalence are clearly evident in conta...
Published on Jun 1, 1972in FEBS Letters2.67
Pierre Jollès47
Estimated H-index: 47
(University of Paris),
J. Berthou10
Estimated H-index: 10
(University of Paris)
Cited By91
Newest
Published on Jul 1, 2017
A. S. Boikova2
Estimated H-index: 2
(RAS: Russian Academy of Sciences),
Yulia A. Dyakova4
Estimated H-index: 4
(RAS: Russian Academy of Sciences)
+ 8 AuthorsYurii V. Pisarevsky1
Estimated H-index: 1
(RAS: Russian Academy of Sciences)
Solutions of lysozyme in heavy water were studied by small-angle neutron scattering (SANS) at concentrations of 40, 20 and 10 mg ml−1 with and without the addition of precipitant, and at temperatures of 10, 20 and 30°C. In addition to the expected protein monomers, dimeric and octameric species were identified in solutions at the maximum concentration and close to the optimal conditions for crystallization. An optimal temperature for octamer formation was identified and both deviation from this ...
Published on Jun 10, 2017
Haruhiko Koizumi5
Estimated H-index: 5
,
Satoshi Uda18
Estimated H-index: 18
+ 2 AuthorsJun Nozawa12
Estimated H-index: 12
Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an...
Published on Nov 1, 2016in Nature Photonics31.58
Yusuke Tominaga1
Estimated H-index: 1
,
Mihoko Maruyama14
Estimated H-index: 14
+ 11 AuthorsKazufumi Takano34
Estimated H-index: 34
The protein crystal growth mechanism can be changed from planar 2D nucleation growth to spiral growth by femtosecond laser ablation. By using this method, the growth rate of a hen egg-white lysozyme crystal increases from 0.3 µm per day to 3.4 µm per day.
Published on Jan 1, 2016in Crystallography Reports0.75
M. A. Marchenkova7
Estimated H-index: 7
(RAS: Russian Academy of Sciences),
V. V. Volkov11
Estimated H-index: 11
(RAS: Russian Academy of Sciences)
+ 6 AuthorsMikhail Kovalchuk7
Estimated H-index: 7
(RAS: Russian Academy of Sciences)
The molecular state of hen egg white lysozyme in solution has been studied by small-angle X-ray scattering (SAXS) combined with molecular simulation. The addition of a precipitant is shown to change the state of the protein molecules in solution. The SAXS data were processed using the constructed models of different oligomers. Under the crystallization conditions, lysozyme is shown to be present in solution as monomers (96.0%), dimers (1.9%), and octamers (2.1%), whereas tetramers and hexamers a...
Published on Oct 7, 2015in Crystal Growth & Design4.15
Jing-Ru Tu3
Estimated H-index: 3
,
Ken-ichi Yuyama9
Estimated H-index: 9
+ 1 AuthorsTeruki Sugiyama16
Estimated H-index: 16
We propose the dynamics and mechanism of laser trapping-induced crystal growth of hen egg-white lysozyme (HEWL). A continuous-wave near-infrared laser beam is used as a trapping light source and focused at a point 10 μm away from a target tetragonal HEWL crystal that is spontaneously generated in solution. Laser trapping of HEWL liquid-like clusters in solution increases local concentration in the focus, where the free motion and orientation of the clusters are strongly restricted, and the clust...
Published on May 6, 2015in Crystal Growth & Design4.15
Yuki Hayashi2
Estimated H-index: 2
,
Mihoko Maruyama14
Estimated H-index: 14
+ 9 AuthorsKazufumi Takano34
Estimated H-index: 34
We measured the normal growth rate of the {110} face (R110) of tetragonal lysozyme crystals grown by the spiral growth mechanism or the two-dimensional (2D) nucleation growth mechanism under various solution flow rates (0, 8.0, 30, 140, 210 μm/s), and observed the step morphology using laser confocal microscopy combined with differential interference contrast microscopy (LCM-DIM). The R110 of crystals grown by the spiral growth mechanism was enhanced by solution stirring at all solution flow rat...
Published on Jun 1, 2014in Chemical Physics Letters1.90
Yoshihiro Takeda13
Estimated H-index: 13
(Tokyo Laboratory),
Fumitaka Mafuné20
Estimated H-index: 20
(UTokyo: University of Tokyo)
Abstract Assembling mechanism of platinum nanoparticles into a {1 0 1} sectors of a tetragonal lysozyme crystal was examined for two different types of the platinum nanoparticles which have either positive or negative ζ potential at pH 3–7. The distribution coefficient of the positive ones inside the crystal is much higher than that of the negative ones in the whole pH range investigated. Dispersivity of the platinum nanoparticles before interaction with the lysozyme crystal surface, which is co...
Published on Dec 1, 2013in FEBS Journal4.74
Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique)
Protein crystallization has been known since 1840 and can prove to be straightforward but, in most cases, it constitutes a real bottleneck. This stimulated the birth of the biocrystallogenesis field with both ‘practical’ and ‘basic’ science aims. In the early years of biochemistry, crystallization was a tool for the preparation of biological substances. Today, biocrystallogenesis aims to provide efficient methods for crystal fabrication and a means to optimize crystal quality for X-ray crystallo...
Published on Feb 1, 2013in Microscopy and Microanalysis2.67
Kathryn Gomery3
Estimated H-index: 3
(UVic: University of Victoria),
Elaine Humphrey6
Estimated H-index: 6
(UVic: University of Victoria),
Rodney Herring9
Estimated H-index: 9
(UVic: University of Victoria)
View next paperGrowth and etching kinetics of tetragonal lysozyme