Match!

Studies of crystal growth mechanisms of proteins by electron microscopy.

Published on Apr 1, 1990in Journal of Molecular Biology5.067
· DOI :10.1016/0022-2836(90)90235-E
S.D. Durbin1
Estimated H-index: 1
(UCSD: University of California, San Diego),
George Feher27
Estimated H-index: 27
(UCSD: University of California, San Diego)
Abstract
Abstract We have used electron microscopy to examine the surfaces of lysozyme crystals and deduce mechanisms of crystal growth. We find that growth occurs by a lattice defect mechanism at low supersaturation and by two-dimensional nucleation at high supersaturation. Step velocities and two-dimensional nucleation rates are obtained, and their dependence on supersaturation is compared with theory. Some features of the observed surface structure can be related to the specific topology and strengths of the bonds in the P 4 3 2 1 2 lattice. Preliminary results on the early stages of nucleation and the phenomenon of cessation of growth are presented.
  • References (13)
  • Citations (92)
📖 Papers frequently viewed together
149 Citations
177 Citations
90 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References13
Newest
#1S.D. Durbin (UCSD: University of California, San Diego)H-Index: 2
#2G. Feher (UCSD: University of California, San Diego)H-Index: 3
177 CitationsSource
#1van Saarloos W (Bell Labs)H-Index: 12
#2George H. Gilmer (Bell Labs)H-Index: 46
Motivated by advances in laser scattering experiments on crystal-melt interfaces during growth conditions, we discuss the interface fluctuations of a faceted crystal during nucleation-dominated growth. If the nucleation rate is large enough so that each layer grows as a result of multiple nucleation events, the properties of long-wavelength interface fluctuations can be understood in terms of a simple random-walk picture. Accordingly, apart from a change in length scale, the interface fluctuatio...
17 CitationsSource
After previous evaporation, growth experiments have been carried out by vapour deposition of NaCl onto NaCl under the following experimental conditions; temperature T=100–350°C, evaporation rate R=0.002–2 nm s-1, and evaporated layer thickness A=0.04–200 nm. Electron microscope observations of step patterns using the gold decoration technique have revealed the great variety of growth modes: random two-dimensional nucleation, hill formation by the spiral mechanism and by repeated two-dimensional ...
20 CitationsSource
#1Luis Bachmann (TUM: Technische Universität München)H-Index: 16
#2R. Becker (TUM: Technische Universität München)H-Index: 1
Last. Wolfgang BaumeisterH-Index: 94
view all 6 authors...
Abstract Aqueous suspensions of catalase crystals were freeze-cleaved, deep-etched and either shadowed with Ta/W at 45° or decorated with 0.1–0.9 nm thick deposits of Au and Pt at normal incidence. The electron micrographs of the decorated specimens were processed by correlation averaging and compared with a relief reconstruction obtained from shadowed specimens. Pronounced decoration was observed on the catalase crystals at temperatures between 130 and 180 K. Disregarding the difficulties in in...
28 CitationsSource
Abstract Transient responses of crystals to the application of a driving force are calculated for three mechanisms of growth. Two-dimensional nucleation is considered in the greatest detail. The transient that results from the nucleation of disk-shaped clusters on a multi-level surface is calcualted by analytical methods. The accuracy of the approximations employed in these methods is assessed by comparison with Monte Carlo results for precisely the same model. Atomistic effects are evaluated us...
73 CitationsSource
#1Zvi Kam (UCSD: University of California, San Diego)H-Index: 42
#2H.B. Shore (SDSU: San Diego State University)H-Index: 1
Last. George Feher (UCSD: University of California, San Diego)H-Index: 27
view all 3 authors...
Abstract We report on theoretical and experimental work aimed at a systematic approach to the crystallization of proteins. Successful crystallization depends on the competition between the growth rates for compact three-dimensional structures and long-chain structures leading to an amorphous precipitate. Quasi-elastic light scattering was used to monitor the size and shape distribution of small aggregates in a model system (lysozyme) during the pre-nucleation stage. With the aid of a simple mode...
214 CitationsSource
#1John Moult (Weizmann Institute of Science)H-Index: 8
#2Ada Yonath (Weizmann Institute of Science)H-Index: 45
Last. A. Saya (Weizmann Institute of Science)H-Index: 1
view all 7 authors...
The gross similarity of the conformation of the hen egg-white lysozyme molecule in the triclinic and tetragonal crystal forms is known from an earlier study. In this work we have established the detailed conformation of the molecule in the triclinic form and compared the two structures using appropriately weighted difference maps. An independent model of the triclinic structure has been obtained by use of the real-space refinement technique. There are appreciable conformational differences of ma...
79 CitationsSource
#1Stephen C. Harrison (Harvard University)H-Index: 109
#2Anthony Abraham Jack (Harvard University)H-Index: 6
The three-dimensional structure of tomato bushy stunt virus has been determined by X-ray crystallographic analysis to 16 A resolution, using methods of multiple isomorphous replacement, together with methods based on non-crystallographic symmetry. The relative orientation of the three quasi-equivalent protein subunits is clearly defined in the map. The geometry of dimer contacts appears to be very similar at strict and local dyads, whereas departures from equivalence are clearly evident in conta...
35 CitationsSource
#1Pierre Jollès (University of Paris)H-Index: 49
#2J Berthou (University of Paris)H-Index: 10
88 CitationsSource
#1S P ColowickH-Index: 1
#2M D JoshiH-Index: 1
Last. N O KaplanH-Index: 1
view all 4 authors...
26k Citations
Cited By92
Newest
#1A. S. Boikova (RAS: Russian Academy of Sciences)H-Index: 2
#2Yulia A. Dyakova (RAS: Russian Academy of Sciences)H-Index: 4
Last. Mikhail V. Kovalchuk (SPbU: Saint Petersburg State University)H-Index: 13
view all 11 authors...
Solutions of lysozyme in heavy water were studied by small-angle neutron scattering (SANS) at concentrations of 40, 20 and 10 mg ml−1 with and without the addition of precipitant, and at temperatures of 10, 20 and 30°C. In addition to the expected protein monomers, dimeric and octameric species were identified in solutions at the maximum concentration and close to the optimal conditions for crystallization. An optimal temperature for octamer formation was identified and both deviation from this ...
8 CitationsSource
#1Haruhiko KoizumiH-Index: 13
#2Satoshi UdaH-Index: 18
Last. Jun NozawaH-Index: 13
view all 5 authors...
Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an...
5 CitationsSource
#1Yusuke TominagaH-Index: 1
#2Mihoko MaruyamaH-Index: 14
Last. Yusuke MoriH-Index: 11
view all 14 authors...
The protein crystal growth mechanism can be changed from planar 2D nucleation growth to spiral growth by femtosecond laser ablation. By using this method, the growth rate of a hen egg-white lysozyme crystal increases from 0.3 µm per day to 3.4 µm per day.
6 CitationsSource
#1M. A. Marchenkova (RAS: Russian Academy of Sciences)H-Index: 7
#2V. V. Volkov (RAS: Russian Academy of Sciences)H-Index: 11
Last. Mikhail V. Kovalchuk (RAS: Russian Academy of Sciences)H-Index: 13
view all 9 authors...
The molecular state of hen egg white lysozyme in solution has been studied by small-angle X-ray scattering (SAXS) combined with molecular simulation. The addition of a precipitant is shown to change the state of the protein molecules in solution. The SAXS data were processed using the constructed models of different oligomers. Under the crystallization conditions, lysozyme is shown to be present in solution as monomers (96.0%), dimers (1.9%), and octamers (2.1%), whereas tetramers and hexamers a...
14 CitationsSource
#1Jing-Ru TuH-Index: 3
#2Ken-ichi YuyamaH-Index: 10
Last. Teruki SugiyamaH-Index: 16
view all 4 authors...
We propose the dynamics and mechanism of laser trapping-induced crystal growth of hen egg-white lysozyme (HEWL). A continuous-wave near-infrared laser beam is used as a trapping light source and focused at a point 10 μm away from a target tetragonal HEWL crystal that is spontaneously generated in solution. Laser trapping of HEWL liquid-like clusters in solution increases local concentration in the focus, where the free motion and orientation of the clusters are strongly restricted, and the clust...
7 CitationsSource
#1Yuki HayashiH-Index: 2
#2Mihoko MaruyamaH-Index: 14
Last. Yusuke MoriH-Index: 11
view all 12 authors...
We measured the normal growth rate of the {110} face (R110) of tetragonal lysozyme crystals grown by the spiral growth mechanism or the two-dimensional (2D) nucleation growth mechanism under various solution flow rates (0, 8.0, 30, 140, 210 μm/s), and observed the step morphology using laser confocal microscopy combined with differential interference contrast microscopy (LCM-DIM). The R110 of crystals grown by the spiral growth mechanism was enhanced by solution stirring at all solution flow rat...
8 CitationsSource
#1Yoshihiro Takeda (Tokyo Laboratory)H-Index: 15
#2Fumitaka Mafuné (UTokyo: University of Tokyo)H-Index: 21
Abstract Assembling mechanism of platinum nanoparticles into a {1 0 1} sectors of a tetragonal lysozyme crystal was examined for two different types of the platinum nanoparticles which have either positive or negative ζ potential at pH 3–7. The distribution coefficient of the positive ones inside the crystal is much higher than that of the negative ones in the whole pH range investigated. Dispersivity of the platinum nanoparticles before interaction with the lysozyme crystal surface, which is co...
3 CitationsSource
#1Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
Protein crystallization has been known since 1840 and can prove to be straightforward but, in most cases, it constitutes a real bottleneck. This stimulated the birth of the biocrystallogenesis field with both ‘practical’ and ‘basic’ science aims. In the early years of biochemistry, crystallization was a tool for the preparation of biological substances. Today, biocrystallogenesis aims to provide efficient methods for crystal fabrication and a means to optimize crystal quality for X-ray crystallo...
66 CitationsSource
#1Kathryn Gomery (UVic: University of Victoria)H-Index: 4
#2Elaine Humphrey (UVic: University of Victoria)H-Index: 6
Last. Rodney A. Herring (UVic: University of Victoria)H-Index: 9
view all 3 authors...
4 CitationsSource