Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with ( R )-MPD had the most order and produced the highest resolution...