Glycosylation of yeast aspartyl—tRNA synthetase

Several lines of evidence establish that the crystallizable aspartyl—tRNA synthetase from Baker's yeast contains some covalently bound glucose: (i) a positive staining of the enzyme was obtained after polyacrylamide gel electrophoresis followed by the concanavalin A‐peroxidase test which is specific for glucose and mannose containing proteins; (ii) thin‐layer chromatography and gas‐liquid chromatography revealed the presence of glucose in enzyme...