Target specificity of the CRISPR‐Cas9 system

The CRISPR‐Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA‐guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off‐target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR‐Cas9...