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Oxidative damage to mitochondria in normal and cancer tissues, and its modulation

Published on Nov 30, 2000in Toxicology3.55
· DOI :10.1016/S0300-483X(00)00279-1
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre),
T.P.A. Devasagayam37
Estimated H-index: 37
(BARC: Bhabha Atomic Research Centre)
Cite
Abstract
Cellular damage induced by reactive oxygen species (ROS) in normal tissues has been implicated in the etiology of several human ailments. Among the subcellular organelles, damage to mitochondria is considered crucial and can lead to cytotoxicity and cell death. However, the same damage, if it is selectively induced in cancer tissues can lead to its cure. Hence analyzing the mechanisms of such damage and its modulation may result in better prevention or cure. Using mitochondria derived from rat brain/liver as well as sarcoma 180 ascites cells, we have examined the mechanisms of damage to lipid, as assessed by different products of lipid peroxidation and to proteins, as determined by loss of enzyme activity and protein oxidation. Mechanisms involved, in terms of scavenging of ROS have been determined using pulse radiolysis for hydroxyl radical and histidine destruction assay for singlet oxygen. Various ROS were generated using γ-radiation, photosensitization etc. under different conditions. Some novel porphyrins, with potential uses in photodynamic therapy also were used as photosensitizers. Our study shows that ROS can induce significant oxidative damage in mitochondria from both normal and tumor tissues and this can be inhibited by natural antioxidants like tocotrienols, nicotinamide and caffeine. Damage, on the other hand, can be enhanced by deuteration of the buffer and oxygenation. Our results hence demonstrated that mitochondria were sensitive to damage by ROS and its modulation may have potential uses in prevention of the disease in normal tissues; if damage can be selectively induced in tumor, it can lead to its regression.
  • References (28)
  • Citations (42)
Cite
References28
Newest
Published on Sep 1, 1997in Molecular and Chemical Neuropathology
Suman K. Mukherjee6
Estimated H-index: 6
(SC: University of Southern California),
James D. Adams15
Estimated H-index: 15
(SC: University of Southern California)
In this work, the tertiary butylhydroperoxide-(t-BuOOH) treated mouse was used as a model to study the oxidative stress that is associated with various neurodegenerative diseases. DNA was found to be an early target oft-BuOOH attack. Necrosis was associated with extensive DNA fragmentation that occurred in almost all regions of the brain within 20 min following intracerebroventricular (icv) injection of 109.7 mg/kgt-BuOOH. Apoptosis was associated with high levels of DNA fragmentation that was o...
Published on Jul 1, 1997in Neuroscience Letters2.17
Germaine Escames15
Estimated H-index: 15
(University of Texas Health Science Center at San Antonio),
Juan Miguel Guerrero Montávez52
Estimated H-index: 52
(University of Texas Health Science Center at San Antonio)
+ 4 AuthorsChang S Oh3
Estimated H-index: 3
(University of Texas Health Science Center at San Antonio)
Abstract We have investigated the level of lipid peroxidation (LPO) in rat brain homogenates in the presence of nitric oxide (NO) which was released by the addition of sodium nitroprusside (SNP) and compared it with that induced by H 2 O 2 . We also examined the effect of melatonin and vitamin E on the NO-induced LPO. The concentration of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was used as an index of LPO. While both H 2 O 2 and SNP increased MDA+4-HDA production in brain homogenates ...
Published on May 1, 1997in Molecular and Cellular Biochemistry2.88
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre),
Haladhar Dev Sarma21
Estimated H-index: 21
(BARC: Bhabha Atomic Research Centre)
+ 2 AuthorsY. Basiron2
Estimated H-index: 2
Tocotrienols from palm oil showed significant ability to inhibit oxidative damage induced by ascorbate-Fe2+ and photosensitization, involving different mechanisms, in rat liver microsomes. The tocotrienol-rich fraction from palm oil (TRF), being tried as a more economical and efficient substitute for α-tocopherol, showed time- and concentration-dependent inhibition of protein oxidation as well as lipid peroxidation. It was more effective against protein oxidation. The extent of inhibition by TRF...
Published on Mar 1, 1997in Photochemistry and Photobiology2.34
Eric E. Kelley29
Estimated H-index: 29
,
Garry R. Buettner66
Estimated H-index: 66
(UI: University of Iowa),
C. Patrick Burns27
Estimated H-index: 27
the photodynamic therapy (PDT)? of cancer (l-3). Photodynamic action relies on the absorption of visible light by the Photofrine photosensitization initiates a sequence of oxidative events that begins with singlet oxygen formation and ultimately leads to cell death. We hypothesize that membrane lipid-derived free radical formation is an early event in this process. In the presence of iron and ascorbate, lipid free radicals are generated during cellular photosensitization of L1210 cells as detect...
Published on Feb 1, 1997in Cancer Letters6.51
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre)
Abstract Hepatic drug metabolizing enzymes were significantly decreased in Ehrlich ascites tumour-bearing mice. A protein inhibitor of hepatic drug metabolizing enzymes was isolated from Ehrlich ascites cells and purified. This involved ammonium sulphate fractionation (60–80%), DEAE, phosphocellulose, Sephadex G-100 and hydroxyapatite column chromatography. Purification attained was 800-fold. The inhibitory protein was effective in decreasing all the components of hepatic mixed-functionoxidase s...
Published on Jan 1, 1997
H. Sies1
Estimated H-index: 1
Natural Antioxidants: B. Halliwell, Antioxidants: The Basics--What They are and How to Evaluate Them. A.M. Bode, Metabolism of Vitamin C in Health and Disease. M.G. Traber, Regulation of Human Plasma Vitamin E. M.E. Anderson, Glutathione and Glutathione Delivery Compounds. L. Packer, S. Roy, and C.K. Sen, a-Lipoic Acid: A Metabolic Antioxidant and Potential Redox Modulator of Transcription. R.J. Reiter, Antioxidant Actions and Melatonin. E. Lovaas, Antioxidative and Metal Chelating Effects of Po...
Published on Jan 1, 1997in Molecular and Cellular Biochemistry2.88
Shampa R. Chatterjee7
Estimated H-index: 7
(IITs: Indian Institutes of Technology),
T. S. Srivastava9
Estimated H-index: 9
(IITs: Indian Institutes of Technology)
+ 1 AuthorsT.P.A. Devasagayam37
Estimated H-index: 37
(BARC: Bhabha Atomic Research Centre)
With a view to locate porphyrins for use in photodynamic therapy (PDT), the new modality of cancer treatment we have evaluated the ability of a novel water soluble porphyrin meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin (T4CPP) to induce damage to mitochondria during photosensitization. T4CPP, when exposed to visible light, induced lipid peroxidation in rat liver mitochondria as assessed by the formation of thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and lipid...
Published on Dec 1, 1996in Cancer Letters6.51
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre)
Chemotherapeutic drugs like cyclophospamide (100 mg/kg b.wt.) and 5-fluorouracil (120 mg/kg b.wt.) caused significant inhibition in cytochrome P450 (cytP450)-dependent hepatic microsomal drug metabolising enzymes in rats. A similar decrease in these enzyme activities was also seen in the host liver of tumour-bearing animals. However, a non-toxic, endogenous (vitamin B3) metabolite, nicotinamide (100 mg/kg b.wt.) could effectively restore the hepatic drug metabolising enzyme levels back to contro...
Published on Nov 1, 1996in Journal of Photochemistry and Photobiology B-biology4.07
Giulio Jori53
Estimated H-index: 53
(UNIPD: University of Padua)
Abstract While Photofrin, the photosensitizer currently in clinical use for photodynamic therapy (PDT) of tumours, has been shown to be both efficacious and safe in the treatment of a variety of human cancers, its chemical heterogeneity and low absorbance in the phototherapeutically useful wavelength range (600–850 nm) make the development of new photosensitizers with improved characteristics desirable. A suitable manipulation of the molecular structure of porphyrins offers several interesting p...
Published on Jun 1, 1996in Biochimica et Biophysica Acta3.79
T.P.A. Devasagayam37
Estimated H-index: 37
(BARC: Bhabha Atomic Research Centre),
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre)
+ 1 AuthorsP.C. Kesavan2
Estimated H-index: 2
(BARC: Bhabha Atomic Research Centre)
Abstract Caffeine (1,3,7-trimethyl xanthine), an ingredient of coffee, has been investigated for its potential antioxidant activity against oxidative damage to rat liver microsomes. Such damage was induced by three reactive oxygen species of cardinal importance in causing membrane damage in vivo namely hydroxyl radical (OH), peroxyl radical (ROO) and singlet oxygen ( 1 O 2 ). The results obtained showed that caffeine was an effective inhibitor of lipid peroxidation, at millimolar concentrations,...
Cited By42
Newest
Published on Jul 17, 2019in FEBS Journal4.74
Xuexue Hu1
Estimated H-index: 1
(CQMU: Chongqing Medical University),
Xiaoqiong Peng1
Estimated H-index: 1
(CQMU: Chongqing Medical University)
+ 8 AuthorsYibing Yin11
Estimated H-index: 11
(CQMU: Chongqing Medical University)
Published on Mar 1, 2019in Chemistry of Natural Compounds0.57
Doan Lan Phuong1
Estimated H-index: 1
(VAST: Vietnam Academy of Science and Technology),
Nguyen Thi Thuy2
Estimated H-index: 2
(H.I., S.I.: University of Agriculture, Faisalabad)
+ 2 AuthorsTran Dinh Thang11
Estimated H-index: 11
(Vinh University)
The present study was aimed at characterizing composition of the seed oils of Afzelia xylocarpa (Kurz) Craib and Cassia fistula L., including the fatty acid profiles and contents of tocopherols, sterols, and total phenolics. The fatty acid profiles of two seed samples were analyzed by GC. The tocopherols and sterols were determined by HPLC, and the total phenolics was assessed by the Folin–Ciocalteau method and HPLC analysis. In addition, the DPPH free radical scavenging assay was used to evalua...
Published on Jan 1, 2019in Carbohydrate Polymers6.04
Selvakumar Palanisamy22
Estimated H-index: 22
(MMU: Manchester Metropolitan University),
Vijayalakshmi Velusamy12
Estimated H-index: 12
(MMU: Manchester Metropolitan University)
+ 3 AuthorsCraig E. Banks60
Estimated H-index: 60
(MMU: Manchester Metropolitan University)
Abstract In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H 2 O 2 biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various p...
Published on Jan 1, 2018in RSC Advances3.05
Si Yao2
Estimated H-index: 2
(Huda: Hubei University),
Yunman Zheng4
Estimated H-index: 4
(WHU: Wuhan University)
+ 7 AuthorsKai Wang2
Estimated H-index: 2
(Huda: Hubei University)
A methylene violet 3RAX-conjugated porphyrin has been designed and synthesized as a potential photosensitizer in photodynamic therapy because of its high DNA binding constant and the efficacy in inhibiting cancer cells with subcellular localization.
Published on Apr 4, 2017in Langmuir3.68
Chongchong Feng6
Estimated H-index: 6
,
Fengyang Wang8
Estimated H-index: 8
+ 3 AuthorsWen Zhang27
Estimated H-index: 27
In this study, a dual-emission fluorescence resonance energy transfer (FRET) polymeric nanoprobe by single-wavelength excitation was developed for sensitive and selective hydrogen peroxide (H2O2) detection. Polymeric nanoprobe was prepared by simple self-assembly of functional lipopolymers, which were 4-carboxy-3-fluorophenylboronic acid (FPBA)-modified DSPE-PEG (DSPE-PEG-FPBA) and 7-hydroxycoumarin (HC)-conjugated DSPE-PEG (DSPE-PEG-HC). Subsequent binding of alizarin red S (ARS) to FPBA endowe...
Published on Oct 1, 2015in Immunology Letters2.55
Michael J. Pecaut22
Estimated H-index: 22
(LLU: Loma Linda University),
Shalini Mehrotra3
Estimated H-index: 3
(LLU: Loma Linda University)
+ 3 AuthorsDaila S. Gridley32
Estimated H-index: 32
(LLU: Loma Linda University)
Abstract There is a growing consensus that long-term deficits in the brain are due to dynamic interactions between multiple neural and immune cell types. Specifically, radiation induces an inflammatory response, including changes in neuromodulatory pro- and anti-inflammatory cytokine secretion. The purpose of this study was to establish that there is sympathetic involvement in radiation-induced decrements early in in vivo immune function host defense. Female, 8–9 week-old C57BL/6J mice were expo...
Published on Sep 1, 2015in Journal of Porphyrins and Phthalocyanines1.29
Zebin Xu3
Estimated H-index: 3
,
Faquan Yu1
Estimated H-index: 1
+ 3 AuthorsXiulan Zhang5
Estimated H-index: 5
Through a new synthetic route, three ruthenium-phenanthroline porphyrins (RPP1, RPP2 and RPP3) were prepared. Their photophysical and photochemical properties, such as DNA photocleavage activity, singlet-oxygen photogeneration and two-photon absorption (2PA) were evaluated. These porphyrins 1–3 had substantial photocleavage activities, with 71%, 74% and 38% observed at 20 μM. The porphyrins with different substituents on phenathroline group had similar singlet oxygen quantum yields, with ΦΔ valu...
Published on Dec 1, 2014in Toxicology and Applied Pharmacology3.58
Jason Z. Li2
Estimated H-index: 2
(VT: Virginia Tech),
Yuebin Ke2
Estimated H-index: 2
(CDC: Centers for Disease Control and Prevention)
+ 4 AuthorsZhenquan Jia23
Estimated H-index: 23
(UNCG: University of North Carolina at Greensboro)
Beta-lapachone (beta-Lp) derived from the Lapacho tree is a potentially novel anticancer agent currently under clinical trials. Previous studies suggested that redox activation of beta-Lp catalyzed by NAD(P)H:quinone oxidoreductase 1 (NQO1) accounted for its killing of cancer cells. However, the exact mechanisms of this effect remain largely unknown. Using chemiluminescence and electron paramagnetic resonance (EPR) spin-trapping techniques, this study for the first time demonstrated the real-tim...
Published on Jan 1, 2013
Satbir Singh1
Estimated H-index: 1
,
Gaurav Sharma + 2 AuthorsAshok Kushnoor4
Estimated H-index: 4
The prevalence of cardiovascular diseases (CVD) like hypertension (HT) is increasing worldwide at an alarming rate. A direct relationship occurs between blood pressure (BP) and the risk of CVD, with oxidative stress as the prime culprit. The evidence which implicates the role of oxidative stress in the pathogenesis and complications of HT suggests the beneficial role of antioxidants in the treatment and prevention of HT. Ample of studies suggest that supplementation with antioxidants play a vita...
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