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Limited Mitochondrial Capacity of Visceral Versus Subcutaneous White Adipocytes in Male C57BL/6N Mice

Published on Mar 1, 2015in Endocrinology3.80
· DOI :10.1210/en.2014-1689
Theresa Schöttl5
Estimated H-index: 5
(TUM: Technische Universität München),
Lisa Kappler2
Estimated H-index: 2
(TUM: Technische Universität München)
+ 2 AuthorsMartin Klingenspor46
Estimated H-index: 46
(TUM: Technische Universität München)
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Abstract
Accumulation of visceral fat is associated with metabolic risk whereas excessive amounts of peripheral fat are considered less problematic. At the same time, altered white adipocyte mitochondrial bioenergetics has been implicated in the pathogenesis of insulin resistance and type 2 diabetes. We therefore investigated whether the metabolic risk of visceral vs peripheral fat coincides with a difference in mitochondrial capacity of white adipocytes. We assessed bioenergetic parameters of subcutaneous inguinal and visceral epididymal white adipocytes from male C57BL/6N mice employing a comprehensive respirometry setup of intact and permeabilized adipocytes as well as isolated mitochondria. Inguinal adipocytes clearly featured a higher respiratory capacity attributable to increased mitochondrial respiratory chain content compared with epididymal adipocytes. The lower capacity of mitochondria from epididymal adipocytes was accompanied by an increased generation of reactive oxygen species per oxygen consumed. Fe...
  • References (50)
  • Citations (10)
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References50
Newest
Published on Jul 1, 2014in Canadian Journal of Zoology1.31
LiYongguo1
Estimated H-index: 1
,
LasarDavid1
Estimated H-index: 1
+ 1 AuthorsKlingensporMartin1
Estimated H-index: 1
Brown fat is a specialized heater organ in eutherian mammals. In contrast to the energy storage function of white adipocytes, brown adipocytes dissipate nutrient energy by uncoupling of mitochondrial oxidative phosphorylation, which depends on uncoupling protein 1 (UCP1). UCP1, as well as UCP2 and UCP3, belong to the family of mitochondrial carriers inserted into the inner mitochondrial membrane for metabolite trafficking between the matrix and the intermembrane space. UCP1 transports protons in...
Published on Jun 1, 2014in Antioxidants & Redox Signaling5.83
Pei-Wen Wang18
Estimated H-index: 18
(CGU: Chang Gung University),
Hsiao-Mei Kuo5
Estimated H-index: 5
(CGU: Chang Gung University)
+ 8 AuthorsTsu-Kung Lin22
Estimated H-index: 22
(CGU: Chang Gung University)
Abstract Aims: Studies in skeletal muscle demonstrate a strong association of mitochondrial dysfunction with insulin resistance (IR). However, there is still a paucity of knowledge regarding the alteration of mitochondria in adipose tissue (AT) in the pathogenesis of IR in obesity. We investigated the mitochondrial biogenesis in visceral fat (VF) and subcutaneous fat (SF) in C57BL/6J mice fed a high-fat high-sucrose diet for 12 months. Results: Impairment of glucose tolerance and insulin sensiti...
Published on Apr 10, 2014in European Journal of Endocrinology5.11
Antonio Giordano20
Estimated H-index: 20
(Marche Polytechnic University),
Arianna Smorlesi16
Estimated H-index: 16
(Marche Polytechnic University)
+ 2 AuthorsSaverio Cinti66
Estimated H-index: 66
(Marche Polytechnic University)
In mammals, adipocytes are lipid-laden cells making up the parenchyma of the multi-depot adipose organ. White adipocytes store lipids for release as free fatty acids during fasting periods; brown adipocytes burn glucose and lipids to maintain thermal homeostasis. A third type of adipocyte, the pink adipocyte, has recently been characterised in mouse subcutaneous fat depots during pregnancy and lactation. Pink adipocytes are mammary gland alveolar epithelial cells whose role is to produce and sec...
Published on Apr 1, 2014in Nature Cell Biology17.73
You-Ying Chau7
Estimated H-index: 7
,
Roberto Bandiera4
Estimated H-index: 4
(University of Nice Sophia Antipolis)
+ 12 AuthorsSophie L. McHaffie2
Estimated H-index: 2
(Edin.: University of Edinburgh)
Increased visceral adipose tissue has been associated with metabolic dysfunction but the origin of the progenitors that give rise to this tissue, and whether they are the same as the progenitors contributing to the protective subcutaneous adipose tissue, was unclear. Hastie and colleagues have found that Wt1-positive mesothelial cells contribute to visceral adipocytes.
Published on Jan 1, 2014in Cardiology Research and Practice
Jaspinder Kaur1
Estimated H-index: 1
Metabolic syndrome is defined by a constellation of interconnected physiological, biochemical, clinical, and metabolic factors that directly increases the risk of cardiovascular disease, type 2 diabetes mellitus, and all cause mortality. Insulin resistance, visceral adiposity, atherogenic dyslipidemia, endothelial dysfunction, genetic susceptibility, elevated blood pressure, hypercoagulable state, and chronic stress are the several factors which constitute the syndrome. Chronic inflammation is k...
Published on Dec 30, 2013in PLOS ONE2.78
Yuko Okamatsu-Ogura14
Estimated H-index: 14
(Hokkaido University),
Keigo Fukano5
Estimated H-index: 5
(Hokkaido University)
+ 4 AuthorsMasayuki Saito47
Estimated H-index: 47
(Tenshi College)
Chronic adrenergic activation leads to the emergence of beige adipocytes in some depots of white adipose tissue in mice. Despite their morphological similarities to brown adipocytes and their expression of uncoupling protein 1 (UCP1), a thermogenic protein exclusively expressed in brown adipocytes, the beige adipocytes have a gene expression pattern distinct from that of brown adipocytes. However, it is unclear whether the thermogenic function of beige adipocytes is different from that of classi...
Published on Apr 1, 2013in Diabetes7.20
Christopher G. R. Perry11
Estimated H-index: 11
(York University),
Daniel A. Kane10
Estimated H-index: 10
(St. Francis Xavier University)
+ 1 AuthorsP. Darrell Neufer39
Estimated H-index: 39
(ECU: East Carolina University)
A growing body of research is investigating the potential contribution of mitochondrial function to the etiology of type 2 diabetes. Numerous in vitro, in situ, and in vivo methodologies are available to examine various aspects of mitochondrial function, each requiring an understanding of their principles, advantages, and limitations. This review provides investigators with a critical overview of the strengths, limitations and critical experimental parameters to consider when selecting and condu...
Published on Dec 1, 2012in Cell Metabolism22.41
Cecile Vernochet12
Estimated H-index: 12
(MPG: Max Planck Society),
Arnaud Mourier21
Estimated H-index: 21
(MPG: Max Planck Society)
+ 14 AuthorsCristina M. Zingaretti4
Estimated H-index: 4
(MPG: Max Planck Society)
Summary Obesity and type 2 diabetes are associated with mitochondrial dysfunction in adipose tissue, but the role for adipose tissue mitochondria in the development of these disorders is currently unknown. To understand the impact of adipose tissue mitochondria on whole-body metabolism, we have generated a mouse model with disruption of the mitochondrial transcription factor A (TFAM) specifically in fat. F-TFKO adipose tissue exhibit decreased mtDNA copy number, altered levels of proteins of the...
Published on Oct 1, 2012in Nature Medicine30.64
Christine M. Kusminski9
Estimated H-index: 9
,
William L. Holland31
Estimated H-index: 31
+ 8 AuthorsCai Li1
Estimated H-index: 1
(MSD: Merck & Co.)
Obesity is often associated with mitochondrial dysfunction. What is not clear, however, is whether this is a cause or a consequence of the condition and its detrimental effects on metabolic health. Phil Scherer and colleagues now show that by manipulating a key protein involved in mitochondrial function specifically in adipocytes the mitochondria is crucial in maintaining proper lipid levels and whole-body insulin sensitivity.
Patrick C. Even26
Estimated H-index: 26
(INRA: Institut national de la recherche agronomique),
Nachiket A. Nadkarni10
Estimated H-index: 10
(Agro ParisTech)
In this article, we review some fundamentals of indirect calorimetry in mice and rats, and open the discussion on several debated aspects of the configuration and tuning of indirect calorimeters. On the particularly contested issue of adjustment of energy expenditure values for body size and body composition, we discuss several of the most used methods and their results when tested on a previously published set of data. We conclude that neither body weight (BW), exponents of BW, nor lean body ma...
Cited By10
Newest
Published on Jul 9, 2019in Frontiers in Physiology3.20
Andrea Kodde2
Estimated H-index: 2
,
Eefje Engels3
Estimated H-index: 3
+ 3 AuthorsJaap Keijer44
Estimated H-index: 44
Published on Mar 26, 2019in Obesity3.97
Britta Wessels (TUM: Technische Universität München), Julius Honecker (TUM: Technische Universität München)+ 4 AuthorsThomas Skurk24
Estimated H-index: 24
(TUM: Technische Universität München)
Published on Jan 1, 2019
Lisa Kappler2
Estimated H-index: 2
,
Laxmikanth Kollipara7
Estimated H-index: 7
+ 1 AuthorsAlbert Sickmann60
Estimated H-index: 60
(Aberd.: University of Aberdeen)
Mitochondrial dysfunction is discussed as a key player in the pathogenesis of type 2 diabetes mellitus (T2Dm), a highly prevalent disease rapidly developing as one of the greatest global health challenges of this century. Data however about the involvement of mitochondria, central hubs in bioenergetic processes, in the disease development are still controversial. Lipid and protein homeostasis are under intense discussion to be crucial for proper mitochondrial function. Consequently proteomics an...
Published on Jun 15, 2018in Frontiers in Physiology3.20
Lorenz Birnbacher8
Estimated H-index: 8
(TUM: Technische Universität München),
Stefanie Maurer10
Estimated H-index: 10
(TUM: Technische Universität München)
+ 3 AuthorsTobias Fromme16
Estimated H-index: 16
(TUM: Technische Universität München)
Phase-contrast computed tomography is an X-ray-based imaging method measuring differences in the refractive index during tissue passage. While conventional X-ray techniques rely on the absorption of radiation due to differing tissue-specific attenuation coefficients, phase-contrast computed tomography enables the determination of the electron density. By the analysis of respective phantoms and ex vivo specimens, we identified the components responsible for different electron densities in murine ...
Published on Apr 1, 2018in Diabetologia7.11
Lily Boutens7
Estimated H-index: 7
(WUR: Wageningen University and Research Centre),
Guido Hooiveld37
Estimated H-index: 37
(WUR: Wageningen University and Research Centre)
+ 3 AuthorsRinke Stienstra29
Estimated H-index: 29
(WUR: Wageningen University and Research Centre)
Aims/hypothesis Recent studies have identified intracellular metabolism as a fundamental determinant of macrophage function. In obesity, proinflammatory macrophages accumulate in adipose tissue and trigger chronic low-grade inflammation, that promotes the development of systemic insulin resistance, yet changes in their intracellular energy metabolism are currently unknown. We therefore set out to study metabolic signatures of adipose tissue macrophages (ATMs) in lean and obese conditions.
Published on Mar 7, 2018in The Journal of Experimental Biology3.02
Theresa Schoettl1
Estimated H-index: 1
,
Ingrid P. Fischer1
Estimated H-index: 1
(TUM: Technische Universität München),
Siegfried Ussar20
Estimated H-index: 20
ABSTRACT Adipose tissue is a central metabolic organ. Unlike other organs, adipose tissue is compartmentalized into individual depots and distributed throughout the body. These different adipose depots show major functional differences and risk associations for developing metabolic syndrome. Recent advances in lineage tracing demonstrate that individual adipose depots are composed of adipocytes that are derived from distinct precursor populations, giving rise to different populations of energy-s...
Published on May 1, 2016
de Andrade Pbm1
Estimated H-index: 1
,
Bolsoni-Lopes A1
Estimated H-index: 1
,
Alonso-Vale Mic1
Estimated H-index: 1
White adipose tissue (WAT) is an endocrine organ with crucial role in the development of obesity and related diseases. White adipocytes have less mitochondria than brown adipocytes; nevertheless, there is an increasing body of evidence showing that mitochondrial parameters play a relevant role in WAT physiology, such as proliferation, differentiation and triacylglycerol storage levels. These parameters comprise mitochondria turnover, oxidative capacity, uncoupling, reactive oxygen species levels...
Published on Feb 1, 2016in Scientific Reports4.01
Lisa Kappler2
Estimated H-index: 2
,
Jia Li12
Estimated H-index: 12
+ 4 AuthorsMiriam Hoene15
Estimated H-index: 15
Subcellular lipidomics is a novel field of research that requires the careful combination of several pre-analytical and analytical steps. To define a reliable strategy for mitochondrial lipid profiling, we performed a systematic comparison of different mitochondria isolation procedures by western blot analyses and comprehensive high-resolution lipidomics. Using liver-derived HepG2 cells, we compared three common mitochondria isolation methods, differential centrifugation (DC), ultracentrifugatio...