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Lectin–carbohydrate interactions on nanoporous gold monoliths

Published on Jan 1, 2013in New Journal of Chemistry3.07
· DOI :10.1039/C3NJ00253E
Yih Horng Tan11
Estimated H-index: 11
(UMSL: University of Missouri–St. Louis),
Kohki Fujikawa11
Estimated H-index: 11
(UMSL: University of Missouri–St. Louis)
+ 4 AuthorsKeith J. Stine20
Estimated H-index: 20
(UMSL: University of Missouri–St. Louis)
Abstract
Monoliths of nanoporous gold (np-Au) were modified with self-assembled monolayers of octadecanethiol (C18-SH), 8-mercaptooctyl α-D-mannopyranoside (αMan-C8-SH), and 8-mercapto-3,6-dioxaoctanol (HO-PEG2-SH), and the loading was assessed using thermogravimetric analysis (TGA). Modification with mixed SAMs containing αMan-C8-SH (at a 0.20 mole fraction in the SAM forming solution) with either octanethiol or HO-PEG2-SH was also investigated. The np-Au monoliths modified with αMan-C8-SH bind the lectin Concanavalin A (Con A), and the additional mass due to bound protein was assessed using TGA analysis. A comparison of TGA traces measured before and after exposure of HO-PEG2-SH modified np-Au to Con A showed that the non-specific binding of Con A was minimal. In contrast, np-Au modified with octanethiol showed a significant mass loss due to non-specifically adsorbed Con A. A significant mass loss was also attributed to binding of Con A to bare np-Au monoliths. TGA revealed a mass loss due to the binding of Con A to np-Au monoliths modified with pure αMan-C8-SH. The use of mass losses determined by TGA to compare the binding of Con A to np-Au monoliths modified by mixed SAMs of αMan-C8-SH and either octanethiol or HO-PEG2-SH revealed that binding to mixed SAM modified surfaces is specific for the mixed SAMs with HO-PEG2-SH but shows a significant contribution from non-specific adsorption for the mixed SAMs with octanethiol. Minimal adsorption of immunoglobulin G (IgG) and peanut agglutinin (PNA) towards the mannoside modified np-Au monoliths was demonstrated. A greater mass loss was found for Con A bound onto the monolith than for either IgG or PNA, signifying that the mannose presenting SAMs in np-Au retain selectivity for Con A. TGA data also provide evidence that Con A bound to the αMan-C8-SH modified np-Au can be eluted by flowing a solution of methyl α-D-mannopyranoside through the structure. The presence of Con A proteins on the modified np-Au surface was also confirmed using atomic force microscopy (AFM). The results highlight the potential for application of carbohydrate modified np-Au monoliths to glycoscience and glycotechnology and demonstrate that they can be used for capture and release of carbohydrate binding proteins in significant quantities.
  • References (113)
  • Citations (13)
References113
Newest
#1Binod Pandey (UMSL: University of Missouri–St. Louis)H-Index: 7
#2Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
view all 6 authors...
#1Zarah Walsh (DCU: Dublin City University)H-Index: 11
#2Brett Paull (DCU: Dublin City University)H-Index: 35
Last.Mirek Macka (UTAS: University of Tasmania)H-Index: 20
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#1Faifan Tantakitti (UW: University of Washington)H-Index: 1
#2Jesse Burk-Rafel (UW: University of Washington)H-Index: 5
Last.Daniel M. Ratner (UW: University of Washington)H-Index: 28
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#1Binod Pandey (UMSL: University of Missouri–St. Louis)H-Index: 7
#2Yih Horng Tan (UMSL: University of Missouri–St. Louis)H-Index: 11
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
view all 5 authors...
Cited By13
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#1Lulu Cai (UNC: University of North Carolina at Chapel Hill)H-Index: 12
#2Zhipeng Gu (SYSU: Sun Yat-sen University)H-Index: 16
Last.Zhen Gu (UNC: University of North Carolina at Chapel Hill)H-Index: 44
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#1Erkin Şeker (UC: University of California)H-Index: 1
#2Wei-Chuan Shih (UH: University of Houston)H-Index: 1
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
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#1Erika Dosekova (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 4
#2Jaroslav Filip (Center for Advanced Materials)H-Index: 12
Last.Jan Tkac (Institute of Chemistry, Slovak Academy of Sciences)H-Index: 32
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#1Abeera Sharma (UMSL: University of Missouri–St. Louis)H-Index: 3
#2Jay K. Bhattarai (UMSL: University of Missouri–St. Louis)H-Index: 4
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
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#1Pavla Bojarová (CAS: Academy of Sciences of the Czech Republic)H-Index: 16
#2Vladimír Křen (CAS: Academy of Sciences of the Czech Republic)H-Index: 48
#1Allan J. Alla (UMSL: University of Missouri–St. Louis)H-Index: 4
#2Felipe B. d’ Andrea (UMSL: University of Missouri–St. Louis)H-Index: 1
Last.Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
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#1Keith J. Stine (UMSL: University of Missouri–St. Louis)H-Index: 20
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