What’s Changed with Genome Editing?

Shengdar Q. Tsai; J. Keith Joung

doi:https://doi.org/10.1016/j.stem.2014.06.017

doi.org/10.1016/j.stem.2014.06.017

What’s Changed with Genome Editing?

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Cell Stem Cell23.90

Volume: 15, Issue: 1, Pages: 3 - 4

Published: Jul 1, 2014

Abstract

Targeted genome editing using engineered nucleases can reliably generate defined genetic mutations, although the range of off-target lesions caused by these techniques is unclear. Recent papers in Cell Stem Cell perform high-coverage whole-genome sequencing to demonstrate the feasibility of obtaining correctly targeted pluripotent stem cell clones with minimal unintended modifications. Targeted genome editing using engineered nucleases can...