Dynamic Screening Experiments to Maximize Hits for Crystallization

In the first step of crystallization screening, the protein is exposed to a wide variety of reagents at different concentrations. Once a “hit” deemed to be conducive to crystallization is identified, parameters such as precipitant concentration, pH, and temperature are used to produce crystals suitable for analysis by X-ray diffraction. Crystals, crystalline precipitate, and phase separation are usually considered leads that are worth pursuing....