Optimizing conditions of polysaccharide extraction from Shiitake mushroom using response surface methodology and its regulating lipid metabolism

Process conditions (extraction time, extraction temperature, water/solid ratio and incubation time) of Shiitake mushroom polysaccharides (SMP) were optimized by conducting experiments at three different levels using the response surface method (RSM). A second-order polynomial response surface equation was developed indicating the effect of variables on polysaccharides yield. Contour maps generated using the response surface equation showed that...