Optimizing conditions of polysaccharide extraction from Shiitake mushroom using response surface methodology and its regulating lipid metabolism

Published on Jun 1, 2013in Carbohydrate Polymers6.044
· DOI :10.1016/j.carbpol.2013.03.035
Min Zhu1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University),
Ping Nie1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
+ 1 AuthorsBing Wang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
Abstract Process conditions (extraction time, extraction temperature, water/solid ratio and incubation time) of Shiitake mushroom polysaccharides (SMP) were optimized by conducting experiments at three different levels using the response surface method (RSM). A second-order polynomial response surface equation was developed indicating the effect of variables on polysaccharides yield. Contour maps generated using the response surface equation showed that all the experimental variables significantly affected the yield. The effect of SMP on oxidative damage in mice fed by high cholesterol diet (HCD) was done in vivo. Results showed that SMP can decreased serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-c) levels, and increased high density lipoprotein (HDL-c) levels in HCD mice. Treatment with SMP reduced blood, liver lipid peroxidation level and increased antioxidant enzymes activities. Thus it can be concluded that SMP can improve lipid metabolism and decreased oxidative damage in HCD mice.
  • References (28)
  • Citations (20)
📖 Papers frequently viewed together
6 Authors (Jianchun Sheng, ..., Qiuhui Hu)
128 Citations
101 Citations
7 Authors (Long-Yan Zhao, ..., Fuhua Zeng)
46 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Ali A. Shati (King Khalid University)H-Index: 6
#2Fahmy G. Elsaid (King Khalid University)H-Index: 6
Last. Elsayed E. Hafez (King Khalid University)H-Index: 13
view all 3 authors...
Abstract Aluminium has been proposed as an environmental factor that may affect several enzymes and other biomolecules related to neurotoxicity and Alzheimer's disease (AD). The promising protective effect of aqueous saffron extract and honey syrup on neurotoxicity induced by aluminuim chloride (AlCl 3 ) may be derived from their own antioxidant properties. Balb/c and C57BL/6 mice (35–40 g) were injected with AlCl 3 , 40 mg/kg/day for 45 days. Each mice strain was divided into four groups: AlCl ...
43 CitationsSource
#1Lina Zhang (WHU: Wuhan University)H-Index: 73
#2Xuelian Li (WHU: Wuhan University)H-Index: 1
Last. Fanbo Zeng (HUST: Huazhong University of Science and Technology)H-Index: 6
view all 4 authors...
Abstract A (1→3)-β- d -glucan having (1→6) branching (L-FV-IB) from Lentinus edodes in water was degraded into seven fractions of different molecular weights by ultrasonic irradiation, and each was further fractionated into three parts, by precipitation from water into acetone at room temperature. The weight-average molecular weight ( M w ), radius of gyration ( S 2 > z 1/2 ), and intrinsic viscosity ([ η ]) of lentinan and its fractions in 0.9% NaCl aqueous solution and dimethyl sulfoxide (Me 2...
172 CitationsSource
#1Patrick H.K. Ngai (CUHK: The Chinese University of Hong Kong)H-Index: 13
#2T.B. Ng (CUHK: The Chinese University of Hong Kong)H-Index: 49
From the fruiting bodies of the edible mushroom Lentinus edodes, a novel protein designated lentin with potent antifungal activity was isolated. Lentin was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and Mono S. The N-terminal sequence of lentin manifested similarity to endoglucanase. Lentin, which had a molecular mass of 27.5 kDa, inhibited mycelial growth in a variety of fungal species including Physalospora piricola, Botrytis cinerea and Mycosphaerella arachidicola. Lentin...
72 CitationsSource
#1Jale Balkan (Istanbul University)H-Index: 14
#2Öznur Kanbagli (Istanbul University)H-Index: 10
Last. Müjdat Uysal (Istanbul University)H-Index: 35
view all 7 authors...
The effect of a high-cholesterol diet with or without taurine on lipids and oxidative stress in the plasma, liver and aorta of rabbits was investigated. The animals were maintained on a basal diet (control), a high-cholesterol diet (HC, 1% w/w), or a high- cholesterol diet supplemented with taurine (HCHT, 2.5% w/w) for two months. Taurine has an ameliorating effect on atherosclerosis together with a decreasing effect on the cholesterol and triglyceride levels in rabbits fed on an HC diet. The HC...
80 CitationsSource
Abstract Malted barley waste produced during the brewing process was used as a medium for the growth of Lentinus edodes mycelium. This by-product proved to be a suitable material for mycelial growth and for the production of high levels of laccase and manganese peroxidase, especially when mixed with oak wood chips. Enzyme production by the mycelium grown on a mixture of malt-containing by-product, oak wood chips and perlite was compared in three fermentation systems. High amounts of enzymes were...
137 CitationsSource
The aim of this study was to investigate the effect of dietary fat levels, with or without cholesterol, on lipid peroxidation and the activities of antioxidant enzymes in rats. Thirty-two Wistar rats aged 4 weeks were divided into 4 groups and fed high (20%; HF) or low (5%; LF) fat, with or without l% cholesterol, for 6 weeks. Cholesterol feeding resulted in significantly higher concentrations of serum cholesterol, but lowered serum triacylglycerol levels. Cholesterol feeding also led to markedl...
19 CitationsSource
#1Mohamedain M. Mahfouz (Urbana University)H-Index: 2
#2Fred A. Kummerow (Urbana University)H-Index: 40
Abstract The objective of this study was to compare the effect of cholesterol feeding of rats and rabbits. The levels of lipid peroxidation products and oxysterols in the plasma of the two species plus the antioxidant enzyme activities in the liver and erythrocytes were measured to explain their different susceptibilities to atherosclerosis. Our study showed that rats are less susceptible than are rabbits to the atherogenic effect of a cholesterol-rich diet because of differences in lipid peroxi...
118 CitationsSource
#1G. Sandhya Devi (Osmania University)H-Index: 2
#2M. Hema Prasad (Osmania University)H-Index: 6
Last. P.P. Reddy (Osmania University)H-Index: 14
view all 6 authors...
Abstract Free radicals are highly reactive species that have been implicated in the pathogenesis of many diseases. Reactive oxygen species can initiate lipid peroxidation and DNA damage leading to mutagenesis, carcinogenesis and cell death, if the antioxidant system is impaired. This study was undertaken to examine the prevalence of oxidative stress and the role of antioxidant defence in untreated leukemia patients. The generation of superoxide anion and hydrogen peroxide by leukocytes, plasma m...
174 CitationsSource
#1Masatomo Hirasawa (Nihon University)H-Index: 23
#2Naoto Shouji (Nihon University)H-Index: 1
Last. Kazuko Takada (Nihon University)H-Index: 18
view all 5 authors...
Three kinds of antibacterial substances were extracted by chloroform, ethylacetate or water from dried Shiitake mushrooms (Lentinus edodes). These substances possess efficient antibacterial activities against Streptococcus spp., Actinomyces spp., Lactobacillus spp., Prevotella spp., and Porphyromonas spp. of oral origin. In contrast, other general bacteria, such as Enterococcus spp., Staphylococcus spp., Escherichia spp., Bacillus spp., and Candida spp. were relatively resistant to these substan...
117 CitationsSource
Abstract Atherosclerosis has often been defined as a multifactoral disease; however, a common risk factor associated with accelerated vascular disease in man or animals is an elevated plasma cholesterol level. Even though there is no one perfect animal model that completely replicates the stages of human atherosclerosis, cholesterol feeding and mechanical endothelial injury are two common features shared by most models of atherosclerosis. The models may differ with respect to degree of dietary c...
58 Citations
Cited By20
#1Tan Liu (NAU: Nanjing Agricultural University)H-Index: 1
#2Jing Peng (NAU: Nanjing Agricultural University)H-Index: 4
Last. Kang Tu (NAU: Nanjing Agricultural University)H-Index: 17
view all 7 authors...
#1Jhonatas Rodrigues Barbosa (UFPA: Federal University of Pará)
#2Maurício Madson dos Santos Freitas (UFPA: Federal University of Pará)H-Index: 1
Last. Raul Nunes de Carvalho Junior (UFPA: Federal University of Pará)H-Index: 5
view all 4 authors...
ABSTRACT The biodiversity of mushrooms Pleurotus spp is impressive due to its complexity and diversity related to the composition of chemical structures such as polysaccharides, glycoproteins and secondary metabolites such as alkaloids, flavonoids and betalains. Recent studies of polysaccharides and their structural elucidation have helped to direct research and development of technologies related to pharmacological action, production of bioactive foods and application of new, more sophisticated...
1 CitationsSource
#1Hongliang Zeng (Fujian Agriculture and Forestry University)H-Index: 11
#2Peilin Chen (Fujian Agriculture and Forestry University)H-Index: 2
Last. Yi Zhang (Fujian Agriculture and Forestry University)H-Index: 15
view all 5 authors...
Abstract The objective was to investigate the hypolipidemic effect of polysaccharides from Fortunella margarita (Lour.) Swingle (FMPS) in hyperlipidemic rats and the comparative relationship between in vitro and in vivo . After FMPS feeding, the body weight, liver and spleen index of the hyperlipidemic rats decreased significantly, in a dose-dependent manner. The content of triglyceride, total cholesterol, low density lipoprotein and serum non-esterified fatty acid decreased, and high density li...
2 CitationsSource
#1Yuyang Lin (Guangdong Pharmaceutical University)H-Index: 2
#2Huiyun Zeng (Guangdong Pharmaceutical University)H-Index: 1
Last. Huajun Fan (Guangdong Pharmaceutical University)H-Index: 8
view all 10 authors...
Abstract Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant. Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH 4 ) 2 SO 4 concentration 19.58% (w/w), extrac...
2 CitationsSource
#1Alicia Gil-Ramírez (Lund University)H-Index: 11
Last. Cristina Soler-Rivas (CSIC: Spanish National Research Council)H-Index: 20
view all 5 authors...
The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3 2 experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending ...
2 CitationsSource
#1Shasha ChengH-Index: 8
#2Ranran LiH-Index: 2
Last. Mingqian TanH-Index: 25
view all 5 authors...
AbstractWater status and distribution in shiitake mushroom and the effects of drying temperature on water migration were investigated by low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) technique. Three water components assigned to bound, immobilized water and free water were observed in fresh shiitake mushroom by LF-NMR, and higher moisture content was found in the lamella and outer layer of stalk by MRI. During drying, the transverse relaxation time and peak a...
2 CitationsSource
#1Zheng Gao (SDAU: Shandong Agricultural University)H-Index: 8
#2Qiangqiang Lai (SDAU: Shandong Agricultural University)H-Index: 2
Last. Le Jia (SDAU: Shandong Agricultural University)H-Index: 19
view all 10 authors...
The antioxidant and multiple organ protection effects of acid- extracted mycelia polysaccharides (Ac-MPS) from Pleurotus eryngii var. tuoliensis on HFE-induced hypertriglyceridemic mice were investigated. The results showed that Ac-MPS have potential ability to relieve the hypertriglyceridemia and preventing oxidative stress by decreasing levels of TG, TC LDL-C, elevating contents of HDL-C in serum, increasing the activities of SOD, GSH-Px, CAT and T-AOC, and the down regulating MDA and LPO cont...
#1Zheng Gao (SDAU: Shandong Agricultural University)H-Index: 8
#2Chen Zhang (SDAU: Shandong Agricultural University)H-Index: 11
Last. Le Jia (SDAU: Shandong Agricultural University)H-Index: 19
view all 10 authors...
Abstract The selenium polysaccharides (SPS) by Oudemansiella radicata were isolated, and two hydrolysates (enzymatic-SPS (ESPS) and acidic-SPS (ASPS)) were obtained by enzymolysis and acidolysis. The endo-toxemic-induced lung and kidney damages were successfully established by the lipopolysaccharides (LPS) injection (5 mg/kg/d). The in vivo mice experiments indicated that the ESPS showed superior antioxidant and protective effects against the LPS-toxicities by increasing the antioxidant activiti...
4 CitationsSource
This study was to investigate the physicochemical properties and anticancer effects of polysaccharides from Lentinus edodes extracted under high pressure cooking treatment (HPLPS) in vitro and in vivo. The extraction efficiency was improved. The main molecular weight of HPLPS was about 540 and about 227 kDa. And the inhibitory effects on HepG2 and HeLa cells of HPLPS were significantly increased (p < 0.05). The in vivo anticancer effect on H22 tumor bearing mice model was evaluated. The tumor gr...
5 CitationsSource
#1Zhendan Ni (ZJU: Zhejiang University)H-Index: 3
#2Shixiang XuH-Index: 1
Last. Tiejin Ying (ZJU: Zhejiang University)H-Index: 21
view all 3 authors...
1 CitationsSource