Match!

Optimizing conditions of polysaccharide extraction from Shiitake mushroom using response surface methodology and its regulating lipid metabolism

Published on Jun 1, 2013in Carbohydrate Polymers6.04
· DOI :10.1016/j.carbpol.2013.03.035
Min Zhu1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University),
Ping Nie1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
+ 1 AuthorsBing Wang2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
Abstract
Abstract Process conditions (extraction time, extraction temperature, water/solid ratio and incubation time) of Shiitake mushroom polysaccharides (SMP) were optimized by conducting experiments at three different levels using the response surface method (RSM). A second-order polynomial response surface equation was developed indicating the effect of variables on polysaccharides yield. Contour maps generated using the response surface equation showed that all the experimental variables significantly affected the yield. The effect of SMP on oxidative damage in mice fed by high cholesterol diet (HCD) was done in vivo. Results showed that SMP can decreased serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-c) levels, and increased high density lipoprotein (HDL-c) levels in HCD mice. Treatment with SMP reduced blood, liver lipid peroxidation level and increased antioxidant enzymes activities. Thus it can be concluded that SMP can improve lipid metabolism and decreased oxidative damage in HCD mice.
  • References (28)
  • Citations (17)
References28
Newest
#1Lina Zhang (WHU: Wuhan University)H-Index: 67
#2Xuelian Li (WHU: Wuhan University)H-Index: 1
Last.Fanbo Zeng (HUST: Huazhong University of Science and Technology)H-Index: 6
view all 4 authors...
#1G. Sandhya Devi (Osmania University)H-Index: 2
#2M. Hema Prasad (Osmania University)H-Index: 6
Last.P.P. Reddy (Osmania University)H-Index: 13
view all 6 authors...
Cited By17
Newest
#1Hongliang Zeng (Fujian Agriculture and Forestry University)H-Index: 11
#2Peilin Chen (Fujian Agriculture and Forestry University)H-Index: 1
Last.Yi Zhang (Fujian Agriculture and Forestry University)H-Index: 13
view all 5 authors...
#1Zheng Gao (SDAU: Shandong Agricultural University)H-Index: 7
#2Qiangqiang Lai (SDAU: Shandong Agricultural University)H-Index: 1
Last.Le Jia (SDAU: Shandong Agricultural University)H-Index: 18
view all 10 authors...
#1Zheng Gao (SDAU: Shandong Agricultural University)H-Index: 7
#2Chen Zhang (SDAU: Shandong Agricultural University)H-Index: 10
Last.Le Jia (SDAU: Shandong Agricultural University)H-Index: 18
view all 10 authors...
#1Diego Morales (CSIC: Spanish National Research Council)H-Index: 1
#2Adriana J. Piris (CSIC: Spanish National Research Council)H-Index: 1
Last.Cristina Soler-Rivas (CSIC: Spanish National Research Council)H-Index: 19
view all 5 authors...
View next paperStudy on antioxidant and anti-aging activities of peptides from sunflower meal