Metabolism of bromhexine in pig hepatocyte cultures

The metabolism of bromhexine [ N ‐cyclohexyl‐ N ‐methyl‐2‐(2‐amino‐3,5‐di‐bromo‐benzyl)‐amine] was studied using pig hepatocyte cultures and LC/MS/MS techniques. Phase I ‘single‐step’ reactions, i.e. hydroxylation and demethylation occurred the fastest whereas the formation of hydroxylated/demethylated and aminal hydroxylated metabolites, which can be considered as multiple‐step reactions, occurred more slowly. Phase II conjugates were detected...