Down‐Regulation of TIMP3 Leads to Increase in TACE Expression and TNFα Production by Placental Trophoblast Cells

Problem To determine whether down‐regulation of TIMP 3 expression promotes TACE expression and increases in TNF α production by placental trophoblast cells. Method of study Placental expression of TIMP 3 and TACE was examined by immunostaining and Western blot. Effects of TIMP 3 on TACE expression and TNF α production were assessed by transfection of TIMP 3 si RNA into trophoblasts isolated from normal placentas. Effects of oxidative stress on...