Thermal phase transition behavior of lipid layers on a single human corneocyte cell

We have improved the selected area electron diffraction method to analyze the dynamic structural change in a single corneocyte cell non-invasively stripped off from human skin surface. The improved method made it possible to obtain reliable diffraction images to trace the structural change in the intercellular lipid layers on a single corneocyte cell during heating from 24 °C to 100 °C. Comparison of the results with those of synchrotron X-ray...