Liquid chromatographic–mass spectrometric analysis of 11 glucocorticoid residues and an optimization of enzymatic hydrolysis conditions in bovine liver
Abstract
A liquid chromatography combined to mass spectrometry (LC–MS/MS) method to confirm the presence of 11 synthetic glucocorticoids in cattle liver is described. After an enzymatic deconjugation step, liver samples were treated with methanol to extract the glucocorticoids. A further cleanup of the extracts was carried out by SPE. LC separations were carried out as described previously using a column packed with porous graphitic carbon. For the...
Paper Details
Title
Liquid chromatographic–mass spectrometric analysis of 11 glucocorticoid residues and an optimization of enzymatic hydrolysis conditions in bovine liver
Published Date
Nov 1, 2002
Journal
Volume
473
Issue
1-2
Pages
127 - 134
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