From osmotic second virial coefficient (B₂₂) to phase behavior of a monoclonal antibody

Antibodies are complex macromolecules and their phase behavior as well as interactions within different solvents and precipitants are still not understood. To shed some light into the processes on a molecular dimension, the occurring self-interactions between antibody molecules were analyzed by means of the osmotic second virial coefficient (B22). The determined B22 follows qualitatively the phenomenological Hofmeister series describing the...