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doi.org/10.1016/j.pep.2009.10.008

Expression plasmids and production of EGFP in stably transfected Acanthamoeba

Erik Bateman
12
Protein Expression and Purification1.60
Volume: 70, Issue: 1, Pages: 95 - 100
Published: Mar 1, 2010
Abstract
New plasmids containing the TATA-Binding Protein (TBP), TBP Promoter Binding Factor (TPBF) or Glyceraldehyde Phosphate Dehydrogenase (GAPDH) gene promoters from Acanthamoeba castellanii are described. The promoters for Acanthamoeba TPBF and GAPDH genes were used to drive constitutive expression of enhanced green fluorescent protein (EGFP) in stably transfected Acanthamoeba. Based initially on fluorescence microscopy and SDS-PAGE analysis of...
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Paper Details
Title
Expression plasmids and production of EGFP in stably transfected Acanthamoeba
DOI
doi.org/10.1016/j.pep.2009.10.008
Published Date
Mar 1, 2010
Journal
Protein Expression and Purification
Volume
70
Issue
1
Pages
95 - 100
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