New method for extending the diffraction pattern from protein crystals and preventing their radiation damage

A CONSIDERABLE problem hampering the analysis of protein crystal structures has been the damage caused to the crystals by the X-ray beam. Another limiting factor has been the resolution in the diffraction pattern. To increase the useful life of a protein crystal in the X-ray beam1, single crystal monochromators and low temperatures have been used. We now report that styrene and other vinyl monomers can decrease the radiation damage of the...