A simple protocol for paraffin‐embedded myelin sheath staining with osmium tetroxide for light microscope observation
Abstract
Experimental investigation of peripheral nerve fiber regeneration is attracting more and more attention among both basic and clinical researchers. Assessment of myelinated nerve fiber morphology is a pillar of peripheral nerve regeneration research. The gold standard for light microscopic imaging of myelinated nerve fibers is toluidine blue staining of resin-embedded semithin sections. However, many researchers are unaware that the dark staining...
Paper Details
Title
A simple protocol for paraffin‐embedded myelin sheath staining with osmium tetroxide for light microscope observation
Published Date
Jul 1, 2008
Volume
71
Issue
7
Pages
497 - 502
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