Maximizing productivity of CHO cell‐based fed‐batch culture using chemically defined media conditions and typical manufacturing equipment

A highly productive chemically defined fed‐batch process was developed to maximize titer and volumetric productivity for Chinese hamster ovary cell‐based recombinant protein manufacturing. Two cell lines producing a recombinant antibody (cell line A) and an Fc‐fusion protein (cell line B) were used for development. Both processes achieved product titers of 10 g/L on day 18 under chemically defined conditions. For cell line B, the use of plant...