Relatively High Levels of Anti-HLA Antibodies (Measured by Luminex Single-Antigen Bead Assay) are Required to Mediate Inhibition of Lymphocyte Proliferation Induced by Sera from Alloimmunized Renal Patients

Rejection of transplanted organs is caused by alloimmune responses, primarily against HLA molecules. Anti-donor HLA antibodies are associated with antibody-mediated rejection (AMR) and poor graft outcome. Because of clinical interest in detecting these antibodies, new technologies have recently been introduced to increase the sensitivity of detection. The Luminex Single-Antigen (LSA) bead assay may yield new information, but it must be validated...