Improved Success of Sparse Matrix Protein Crystallization Screening with Heterogeneous Nucleating Agents

Published on Oct 31, 2007in PLOS ONE2.78
· DOI :10.1371/journal.pone.0001091
Anil S. Thakur3
Estimated H-index: 3
(UQ: University of Queensland),
Gautier Robin10
Estimated H-index: 10
(ARC: Australian Research Council)
+ 4 AuthorsBostjan Kobe52
Estimated H-index: 52
(UQ: University of Queensland)
Crystallization is a major bottleneck in the process of macromolecular structure determination by X-ray crystallography. Successful crystallization requires the formation of nuclei and their subsequent growth to crystals of suitable size. Crystal growth generally occurs spontaneously in a supersaturated solution as a result of homogenous nucleation. However, in a typical sparse matrix screening experiment, precipitant and protein concentration are not sampled extensively, and supersaturation conditions suitable for nucleation are often missed.
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