A highly productive system for cell-free protein synthesis using a lysate of the hyperthermophilic archaeon, Thermococcus kodakaraensis

We report in this study an improved system for cell-free protein synthesis at high temperatures using the lysate of Thermococcus kodakaraensis. Previous work indicated that cell-free protein synthesis of ChiAΔ4, a derivative of T. kodakaraensis chitinase, was observed within a temperature range of 40–80°C, and the maximum yield of the ChiAΔ4 synthesized was approximately 1.3 μg/ml. To increase productivity of the system, the following approaches...