Analysis of free, mono- and diacetylated polyamines from human urine by LC–MS/MS

Polyamines are promising biochemical markers of cancer and many other pathophysiological conditions, and thus their concentrations in biological fluids are a matter of interest. However, since the concentrations of these compounds are low, their quantitation is typically based on methods requiring laborious sample preparation. Here we developed and validated an LC–MS/MS method to analyze simultaneously free (DAP, PUT, CAD, SPD, SPM)...